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Merck

D8662

Sigma-Aldrich

杜氏磷酸盐缓冲盐水

Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture

别名:

DPBS

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
eCl@ss:
32129211
NACRES:
NA.75

产品名称

杜氏磷酸盐缓冲盐水, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

sodium pyruvate: no
glucose: no (Dextro)

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

杜氏磷酸盐缓冲盐水(D-PBS)是一种用于组织培养的平衡盐溶液。

應用

D-PBS是通用的等渗盐水溶液,用于在制备和连续转移过程中洗涤细胞。
杜氏磷酸盐缓冲盐水(D-PBS)已用于:
  • 作为前神经生长因子(proNGF)-YBBR荧光标记反应混合物的组分
  • 在Caco-2BBe细胞经上皮电阻(TEER)测量后清洗和平衡细胞
  • 清洗细胞以进行蛋白质分离

杜氏磷酸盐缓冲盐水(D-PBS)已用于:
  • 作为前神经生长因子(proNGF)-YBBR荧光标记反应混合物的组分
  • 在Caco-2BBe细胞经上皮电阻(TEER)测量后清洗和平衡细胞
  • 清洗细胞以进行蛋白质分离

生化/生理作用

杜氏磷酸盐缓冲盐水(D-PBS)有四种主要用途:
  • 为细胞提供正常细胞代谢所必需的水分和一定体积的无机离子
  • 充当灌溉、运输和稀释液体,同时维持细胞内和细胞外渗透平衡
  • 与碳水化合物(如葡萄糖)结合时,为细胞代谢提供主要的能量来源
  • 提供缓冲系统,以维持培养基的生理pH值范围

相關產品

产品编号
说明
价格

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
To compare the capsulotomy rim strength with capsular marks (CMs) to the rim strength without CMs in porcine eyes, and to demonstrate the practicality of CMs for intraoperative toric intraocular lens (IOL) alignment. LENSAR facility, Orlando, Florida, USA. Laboratory study.
L Parmegiani et al.
Journal of assisted reproduction and genetics, 35(10), 1887-1895 (2018-08-04)
To study the efficacy and efficiency of a "universal warming protocol" for vitrified human embryos, based on subsequent steps with 1 and 0.5 M concentration of extracellular cryoprotectant (ECCP). Two studies on patients undergoing fertility treatments via ICSI: a prospective randomized
Michele Russo et al.
European journal of heart failure, 21(4), 519-528 (2019-03-08)
Butyric acid (BUT), a short chain fatty acid produced daily by the gut microbiota, has proven beneficial in models of cardiovascular diseases. With advancements in cancer survival, an increasing number of patients are at risk of anticancer drug cardiotoxicity. Here
Ivan Kopljar et al.
British journal of pharmacology, 174(21), 3766-3779 (2017-01-18)
In the pharmaceutical industry risk assessments of chronic cardiac safety liabilities are mostly performed during late stages of preclinical drug development using in vivo animal models. Here, we explored the potential of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) to
Kimberly R Long et al.
Molecular biology of the cell, 28(19), 2508-2517 (2017-07-20)
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular

实验方案

制备3D癌症干细胞球状体的分步肿瘤成球实验方案

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。

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