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Merck

11681451001

Roche

NBT/BCIP 储存液

别名:

5-溴-4-氯-3-吲哚磷酸, BCIP

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About This Item

分類程式碼代碼:
12352204

形狀

solution

品質等級

分子量

817.7

包裝

pkg of 8 mL

製造商/商標名

Roche

儲存溫度

2-8°C

相关类别

一般說明

反应产物呈蓝色,不溶于水。蓝色为肉眼可见。

配方:NBT:C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N

评估: 肉眼

應用

  • 该溶液用于通过印迹方案灵敏检测碱性磷酸酶(AP),适用于:DNA印迹
  • RNA印迹
  • 蛋白质印迹
  • 菌落及菌斑清除
  • 原位杂交
  • 以及免疫组织化学和免疫化学应用
注: NBT/BCIP可同时用于硝酸纤维膜和尼龙膜。

外觀

溶液中含硝基蓝四唑氯铵18.75 mg/ml, 5-溴-4-氯-3-吲哚基磷酸盐9.4 mg/ml,甲苯胺盐67% (DMSO) (v/v)。
注: 溶液颜色可能从黄色到淡棕色不等。

準備報告

工作溶液:制备染色液
该溶液需临使用前配制。
  • 除DIG体系外的其他应用:向10 ml的 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2中加入200 μl的储备溶液。
  • DIG体系应用:向10 ml的 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl中加入200 μl的储备溶液
注:检测缓冲液中不能含MgCl2,否则会导致检测操作步骤后在膜上呈现斑点背景。不溶于水。

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


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