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磷酸钙转染试剂盒

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

别名:

Gene delivery

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About This Item

EC號碼:
233-140-8
分類程式碼代碼:
12352200
NACRES:
NA.85

等級

for molecular biology

品質等級

200

形狀

solution

用途

 kit sufficient for 160 transfections (6 cm dishes)
 kit sufficient for 400 transfections (3.5 cm dishes)
 kit sufficient for 80 transfections (10 cm dishes)

技術

transfection: suitable

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

一般說明

磷酸钙法转染是将DNA引入真核细胞的常用方法。这一技术已经在广泛的细胞类型中使用，可实现瞬时和稳定的转染效果。

應用

磷酸钙转染试剂盒用于：
  • 启动转染
  • 转染 Hek293T 细胞
  • 转染 H29D 细胞

适用于瞬时和稳定地将DNA转染至培养的哺乳动物细胞中。 使用磷酸钙方法已成功转染了以下细胞：

BAEC
肠黑素瘤细胞
CHO K1
COS-7
成纤维细胞（人胚胎、新皮肤）
HEK293
Huh7
IMR-90
LLC（Lewis肺癌）
NIH3T3
PC-12
PCI-13
SH-SY5Y
SK-HEP-1
T47D

特點和優勢

  • 适用于瞬时和稳定转染
  • 可重复用于多种细胞类型
  • 广泛引用
  • 价格便宜

成分

磷酸钙转染试剂盒包含：
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES 缓冲盐 (H1012)
25 ml 分子生物学级纯水(W4502)

原則

该方法是基于将含有磷酸钠的HEPES缓冲盐水和含有DNA的CaCl2 溶液进行缓慢混合实现的。 所形成的DNA-磷酸钙共沉淀物可粘附于细胞表面，并可能通过胞吞作用被细胞吸收。

其他說明

该方案可用于使用pSV40-CAT质粒作为报告基因对CHO细胞进行瞬时转染。

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H319

危險分類

Eye Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Munjin Kwon et al.
Methods in molecular biology (Clifton, N.J.), 1018, 107-110 (2013-05-18)
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA-calcium phosphate precipitates are internalized by the cells and DNA is efficiently expressed in
M Wigler et al.
Cell, 14(3), 725-731 (1978-07-01)
Previous studies from our laboratories have demonstrated the feasibility of transferring the thymidine kinase (tk) gene from restriction endonuclease-generated fragments of herpes simplex virus (HSV) DNA to cultured mammalian cells. In this study, high molecular weight DNA from cells containing
A new technique for the assay of infectivity of human adenovirus 5 DNA.
F L Graham et al.
Virology, 52(2), 456-467 (1973-04-01)
Denise A Carbonaro et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 22(3), 607-622 (2013-11-22)
Gene transfer into autologous hematopoietic stem cells by γ-retroviral vectors (gRV) is an effective treatment for adenosine deaminase (ADA)-deficient severe combined immunodeficiency (SCID). However, current gRV have significant potential for insertional mutagenesis as reported in clinical trials for other primary
Use of retroviral-mediated gene transfer to deliver and test function of chimeric antigen receptors in human T-cells
Parente-Pereira A C, et al.
Journal of biological methods, 1(2), e7-e7 (2014)
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商品

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

细胞转染简介

转染是将DNA、RNA或蛋白质引入真核细胞的过程，用于研究和调节基因表达。因此，转染技术和实验方案作为分析工具，有助于表征遗传功能、蛋白质合成、细胞生长和发育。

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

实验方案

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

磷酸钙转染试剂盒实验方案

使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。

相关内容

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

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