Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
(NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
• Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
• While gently stirring the water, add 100 ml of 10X medium.
• To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
• While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
• Bring medium to final volume with additional tissue culture grade water.
• It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
• Aseptically dispense into sterile containers. Store refrigerated at 2-8° C
M9288
Medio esencial mínimo Eagle
With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x
Sinónimos:
MEM
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32,60 €
32,60 €
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100 ML
32,60 €
About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
32,60 €
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Nombre del producto
Medio esencial mínimo Eagle, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Nivel de calidad
esterilidad
sterile-filtered
Formulario
liquid
concentración
10 ×
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
componentes
NaHCO3: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
HEPES: no
sodium pyruvate: no
phenol red: yes
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
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Descripción general
Minimum Essential Medium (MEM), one of the most used synthetic cell culture media, is developed by Harry Eagle. It is used to cultivate various types of cells grown in monolayers.
Aplicación
Minimum Essential Medium Eagle has been used as a transport media to maintain pig liver cells.[1]
Reconstitución
Supplement with 0.292 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.
suplemento
Referencia del producto
Descripción
Precios
también adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?
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