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M0275

Medio esencial mínimo Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Sinónimos:

MEM

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A ustedes/SKUDisponibilidadPrecio
100 mL
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30,20 €
500 mL
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71,90 €

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UNSPSC Code:
12352207
NACRES:
NA.71

30,20 €

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Nombre del producto

Medio esencial mínimo Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel).[1] It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.

Preparation Note

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

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1 of 1

Este artículo
M8167M2645M8028
Medio esencial mínimo Eagle 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M0275

Medio esencial mínimo Eagle

Medio esencial mínimo Eagle Spinner Modification, with Earle′s salts and sodium bicarbonate, without calcium chloride and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M8167

Medio esencial mínimo Eagle

Medio esencial mínimo Eagle HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

SAFC

M2645

Medio esencial mínimo Eagle

Joklik Modified MEM Joklik Modification, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M8028

Joklik Modified MEM

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

form

liquid

form

liquid

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: yes
L-glutamine: no
HEPES: no

components

HEPES: 25 mM
phenol red: yes
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes

components

glucose: yes
phenol red: yes
HEPES: no
Hanks’ salts (2% CO2): no
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): no
sodium pyruvate: no
stable glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Preguntas

  1. If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?

    1 respuesta

    1. Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
      Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
      (NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
      • Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
      • While gently stirring the water, add 100 ml of 10X medium.
      • To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
      • While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
      • Bring medium to final volume with additional tissue culture grade water.
      • It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
      • Aseptically dispense into sterile containers. Store refrigerated at 2-8° C

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