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Merck

S5761

Sigma-Aldrich

Bicarbonato de sodio

Sodium bicarbonate

powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Sinónimos:

Sodium hydrogen carbonate, soda bicarbonate

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500 G
61,10 €
1 KG
108,00 €
5 KG
363,00 €

61,10 €


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500 G
61,10 €
1 KG
108,00 €
5 KG
363,00 €

About This Item

Fórmula lineal:
NaHCO3
Número de CAS:
Peso molecular:
84.01
Beilstein:
4153970
Número CE:
Número MDL:
Código UNSPSC:
12161702
ID de la sustancia en PubChem:
NACRES:
NA.75
Ensayo:
99.5-100.5%
grado:
for molecular biology
Formulario:
powder
solubilidad:
water: soluble 100 mg/mL

61,10 €


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grado

for molecular biology

Nivel de calidad

Línea del producto

BioReagent

Ensayo

99.5-100.5%

Formulario

powder

calidad

300

técnicas

cell culture | insect: suitable
cell culture | mammalian: suitable

pH

6.8-7.4

pKa (25 °C)

(1) 6.37, (2) 10.25 (carbonic acid)

solubilidad

water: soluble 100 mg/mL

densidad

2.16 g/mL at 25 °C (lit.)

cadena SMILES

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

Clave InChI

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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Descripción general

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.

Aplicación

Sodium bicarbonate has been used:
  • as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)[1]
  • as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells[2]
  • as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells[3]
  • as a component of medium used for cell culture of human embryonic kidney cells[4]
  • as a component of Beltsville thawing solution used for the dilution of semen[5]

Producto relacionado

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Preguntas

1–2 de 2 Preguntas  
  1. What is the size of his powder?

    1 respuesta
    1. The particle size of the powder has not been measured.

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  2. What is the shelf life after delivery and opening? Is there usually an impact on quality after 2 years? The color of the medium changed and the pH decreased. Does the solubility or function of sodium bicarbonate decrease as it gets older?

    1 respuesta
    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 5 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      After opening, various factors may influence the performance of the product. Moisture uptake and cross-contamination are common issues that are dependent on product handling and storage. If the product you are using is experience stability issues before the recommended restest date, this reporting should be investigated by our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

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