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M2279

Sigma-Aldrich

Medio esencial mínimo Eagle

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

Sinónimos:

MEM

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

product name

Medio esencial mínimo Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Nivel de calidad

400

esterilidad

sterile-filtered

formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera.

Aplicación

Minimum Essential Medium Eagle has been used to culture the:
  • explants of aortic valve leaflets
  • human liver cancer cell line (HepG2) cells
  • human fetal brain progenitor-derived astrocyte cells (PDA)
  • breast cancer cell line (MCF-7)
  • human glioblastoma cell line (T98G)

Nota de preparación

Supplement with 0.292 g/L L-glutamine.

Opcional

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Qingyun Li et al.
Neuron, 101(2), 207-223 (2019-01-05)
Microglia are increasingly recognized for their major contributions during brain development and neurodegenerative disease. It is currently unknown whether these functions are carried out by subsets of microglia during different stages of development and adulthood or within specific brain regions. Here
Winston X Yan et al.
Nature communications, 8, 15058-15058 (2017-05-13)
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along the genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ and Sequencing (BLISS)
Kaisa-Emilia Makkonen et al.
Journal of virology, 87(20), 11148-11159 (2013-08-09)
Baculoviruses are insect-specific viruses commonly found in nature. They are not able to replicate in mammalian cells but can transduce them when equipped with an appropriate mammalian cell active expression cassette. Although the viruses have been studied in several types
Emily R Trzeciak et al.
Cells, 11(6) (2022-03-26)
The cellular composition of the tumor microenvironment, including tumor, immune, stromal, and endothelial cells, significantly influences responses to cancer therapies. In this study, we analyzed the impact of oxidative stress, induced by cold atmospheric plasma (CAP), on tumor cells, T
Luciano A Masullo et al.
Nature communications, 9(1), 3281-3281 (2018-08-18)
The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging
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