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B3883

Millipore

Bile bovine

dried, unfractionated

Sinónimos:

Ox gall powder

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About This Item

Número de CAS:
8008-63-7
EC Number:
232-369-0
MDL number:
MFCD00130641
UNSPSC Code:
41106212
NACRES:
NA.85
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sterility

non-sterile

Quality Level

200

form

dried, unfractionated

color

yellow-brown

solubility

water: soluble 50 mg/mL

application(s)

microbiology

General description

Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.

Application

Bile bovine has been used:
  • in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition[1]
  • as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology[2]
  • as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583[3]

Biochem/physiol Actions

Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.[4]

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000421105
0000419447
0000419447
0000416614
0000416614

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Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties
Guo XH, et al.
Anaerobe, 16(4), 321- 326 (2010)
Bile acid patterns in commercially available oxgall powders used for the evaluation of the bile tolerance ability of potential probiotics
Hu PL, et al.
PLoS ONE, 13(3), e0192964-e0192964 (2018)
Transcriptional responses of Enterococcus faecalis V583 to bovine bile and sodium dodecyl sulfate
Solheim M, et al.
Applied and Environmental Microbiology, 73(18), 5767- 5774 (2007)
Helena Šeme et al.
Food research international (Ottawa, Ont.), 94, 45-53 (2017-03-16)
Malabsorption of dietary sugars is a common cause of gastrointestinal discomfort, affecting up to one in three people with debilitating symptoms, such as abdominal pain, osmotic diarrhoea, bloating and flatulence. Besides dietary interventions, it has been suggested that ingestion of
Impact of bile salt adaptation of Lactobacillus delbrueckii subsp. lactis 200 on its interaction capacity with the gut
Burns P, et al.
Research in Microbiology, 162(8), 782- 790 (2011)
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Questions

1–3 of 3 Questions  

  1. WHAT IS THE PROPER TEMPERATURE FOR STORAGE?

    1 answer

    1. Item B3883 can be stored at room temperature.

      Helpful?

  2. Can you tell us the exact bile salt concentration?

    1 answer

    1. This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however other components such as glycocholic and taurocholic acid are also present.

      Helpful?

  3. I need to prepare bile salt (10 mM). May I know the content of Bile in this product? Or the molecular weight of this products? Thanks.

    1 answer

    1. This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however, other components such as glycocholic and taurocholic acid are also present.

      Helpful?

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