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70168

Millipore

Ox-bile, dehydrated, purified

suitable for microbiology, extracted under controlled conditions from purified fresh bile

Sinónimos:

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.85

Quality Level

sterility

non-sterile

form

powder

impurities

~5% sodium chloride

ign. residue

≤22% (as SO4)

loss

≤8% loss on drying

pH

6.0-9.2 (2% in H2O)

solubility

distilled water: freely soluble

application(s)

microbiology

General description

Ox-bileis a dehydrated, purified fresh bile which is used as a selective inhibitory agent in bile media. Ox-bile desiccated is prepared by a low temperature dehydration process which ensures a uniform product and eliminates the difficulties encountered in obtaining satisfactory supplies of fresh bile. An 8 to 10% solution of the dehydrated product is equivalent to fresh bile. 1 gram of dried Ox Bile is equivalent to 8 grams of fresh bile.

Application

Ox bile is used as a selective agent in culture media for selective growth of intestinal bacteria like E. coli, other feacal coliforms, feacal streptococci. But it is also used as a selective agent in media for detection of Yersinia enterocolitica, Aeromonas hydrophila and Vibrio species. Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells. It may also be used for identification of isolates of Vibrio vulnificus (Beneckea) which is a halophilic bacterium, found responsible for causing several human infections.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Functional properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
Maldonado N, et al.
International Journal of Veterinary Science & Research, 1(1), 011-022 (2015)
Gareth A Williams et al.
PloS one, 14(4), e0214859-e0214859 (2019-04-20)
In developing an oral bait BCG vaccine against tuberculosis in badgers we wanted to understand the conditions of the gastrointestinal tract and their impact on vaccine viability. Conditions mimicking stomach and small-intestine caused substantial reduction in BCG viability. We performed

Artículos

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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