PDB information is not available for this product.
L3126
Lipase from porcine pancreas
Lipase from porcine pancreas
Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Sinónimos:
Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
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About This Item
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Productos recomendados
origen biológico
Porcine pancreas
tpo
Type II
Formulario
lyophilized (cake)
actividad específica
≥125 units/mg protein (using olive oil (30 min incubation))
30-90 units/mg protein (using triacetin)
composición
protein, ≥20%
temp. de almacenamiento
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
Clave InChI
QWZUIMCIEOCSJF-CHHCPSLASA-N
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Aplicación
Lipase from porcine pancreas has been used:
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Acciones bioquímicas o fisiológicas
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Calidad
Crude (Steapsin). Contains amylase and protease activity.
Definición de unidad
One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)
Nota de análisis
Protein determined by biuret.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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Artículos
Lipid Induced Insulin Resistance
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May I know the PDB information related to this lipase protein?
1 answer-
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I want to use this product (L3126) for preparing a solution to conduct in vitro degradation experiments on a particular material. Could you please provide detailed instructions on how to prepare the solution, including any recommendation?
1 answer-
Unfortunately, there is no application specific instructions available for this product. The material may be reconstituted in 100 mM potassium phosphate buffer, pH 8.0, containing 1 mg/ml BSA. This product is from porcine pancreas. Another product option, L3419 Lipase from Thermus thermophilus, is reconstituted in 50 mM Tris-HCl, pH 8.0. This material states that solutions are stable for up to 1 month at 2 - 8°C. Please see the link below to review the datasheet for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/504/l3419dat.pdfHelpful?
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How is a "microequivalent" of fatty acid quantified in the enzymatic activity unit definition for L3126?
1 answer-
A microequivalent is equivalent to 1 mmol/L. In this case, one unit of Lipase will hydrolyze 1.0 microequivalent (1 mmol/L) of fatty acid from olive oil in 1 hour at pH 7.7 at 37°C.
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