No, this product does not contain a His-tag. See the link below for additional product information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/394/024/p7582dat.pdf
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P7582
Amino-terminal FLAG-BAP™ Fusion Protein
Synonym(e):
Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein
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100 μG
338,00 €
338,00 €
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100 μG
338,00 €
About This Item
338,00 €
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Empfohlene Produkte
Form
liquid
Mol-Gew.
~49 kDa
Versandbedingung
dry ice
Lagertemp.
−20°C
Verwandte Kategorien
Allgemeine Beschreibung
Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.[1]
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.
Anwendung
Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification[2] and in western blotting based quantification of Flag-Wnt8[3] and Drosophila melanogaster transcription factor interactome.[4]
Learn more product details in our FLAG® application portal.
Sonstige Hinweise
Control protein
Physikalische Form
Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.
Rechtliche Hinweise
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Kunden haben sich ebenfalls angesehen
BAP-fusion: A versatile molecular probe for biotechnology research
Biotechnology: Research, Technology and Applications, 327-345 (2008)
A Comprehensive Drosophila melanogaster Transcription Factor Interactome
Shokri L, et al.
Cell Reports, 27(3), 955-970 (2019)
Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis
Eroshkin FM, et al.
Scientific Reports, 6, 23049-23049 (2016)
Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates
Guo S, et al.
Biotechnology Progress, 35(1), e2722-e2722 (2019)
Daniela Castiglia et al.
Biotechnology for biofuels, 9, 154-154 (2016-07-28)
Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of the present study was to explore the production in tobacco plastome of
Artikel
Vergleich von Elutionstechniken für die Proteinaufreinigung von FLAG® Tag Proteinen im kleinen Maßstab mittels anti-FLAG® M2 Magnetperlen.
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
Protokolle
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
Protokoll für die Immunpräzipitation (IP) von FLAG-Fusionsproteinen unter Einsatz des monoklonalen M2-Antikörper-Affinitätsgels (4 % Agarose)
Verwandter Inhalt
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Proteinaufreinigungsverfahren, Reagenzien und Protokolle zur Aufreinigung rekombinanter Proteine unter Anwendung von Methoden wie Ionenaustausch, Größenausschluss und Proteinaffinitätschromatographie.
Proteinexpressionstechnologien für verschiedene Expressionssysteme zur Unterstützung von Forschung und der Herstellung von Arzneimitteln und Impfstoffen.
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
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Does the FLAG-BAP fusion protein also contain a his tag?
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