For a list of components, see the link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/747/307/d8437for.pdf
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D8437
DMEM/F-12
Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham
With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym(e):
DME/F-12, 1:1 Mischung
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500 ML
48,30 €
About This Item
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Produktbezeichnung
Dulbecco Modifiziertes Eagle-Medium/Nährstoffmischung F-12 Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Qualitätsniveau
Sterilität
sterile-filtered
Form
liquid
Methode(n)
cell culture | mammalian: suitable
Verunreinigungen
endotoxin, tested
Komponenten
glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L
Versandbedingung
ambient
Lagertemp.
2-8°C
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Allgemeine Beschreibung
Dulbecco′s Modified Eagle′s Medium (DMEM), das regelmäßig als Zellkulturmedium verwendet wird, ist eine Quelle für energiereiche Medien, die Glucose, Vitamine und Aminosäuren umfasst.[1] Eine 1:1-Mischung von Dulbecco′s Modified Eagle′s Medium (DME) und Hams F-12-Nährstoffmischung wird verbreitet in der Zellkulturforschung eingesetzt. Um den durch das Weglassen des Serums ausgelösten Verlust der Pufferkapazität auszugleichen, kann den Formulierungen eine Endkonzentration von 15 mmol/l 4-(2-Hydroxyethyl)-1-piperazinethansulfonsäure(HEPES)-Puffer zugegeben werden.
Anwendung
Dulbecco′s Modified Eagle′s Medium/Nährstoffmischung F-12 nach Ham:
- wurde als Komponente im Kulturmedium zum Kultivieren von primären kulturaktivierten humanen Lebersternzellen eingesetzt[1]
- wurde zum Kultivieren von Knorpelbiopsien eingesetzt[2]
- kann zum Propagieren von Cowdria ruminantium eingesetzt werden[3]
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Artikel
Nutrient Mixture F-12 Ham Formulation
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Dear, Can you tell me the composition of this medium, or at least the amount of L-Glutamine present in this media? Kind regards, Sarah
1 Antwort-
Hilfreich?
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Is the glutamine that has already been added stable glutamine? How long is the shelf life?
1 Antwort-
This product does not contain Alanyl-glutamine, which is considered a more stable form It has been supplemented with L-glutamine and will remain stable through out the shelf life of the product with proper storage and handling. Typical lots of D8437 are assigned an expiration date of 1 year from the date of release. Note that under culture conditions of 37 deg C the L-glutamine will gradually degrade. See the link below to review general stability information regarding L-glutamine.:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/195/924/t075.pdfAlanyl-glutamine is also available as a sterile medium supplement. Please see the link below for an example of one cell culture product option:
https://www.sigmaaldrich.com/product/sigma/g8541Hilfreich?
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Can Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, be used under serum free conditions?
1 Antwort-
When DME/F12 is used under serum free conditions, supplementation is necessary. DME/F12 can be used for growing the epithelial cell line TM4 (mouse testes) when supplemented with insulin, transferrin, EGF, FSH, somatomedin C, ovine growth hormone, and retinoic acid. Primary cultures of Sertoli cells can also be grown in the above supplemented media. Rat glial C6 cells have been grown in DME/F12 supplemented with transferrin, insulin, LH, and rat submaxillary gland extract. See: Jakoby, W.B., and Pastan, I.H., Cell Culture, Methods in Enzymology, 58, 103-106 (1979). (This book is listed as Product No. C0541).
Hilfreich?
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Hilfreich?
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What is the optimal carbon dioxide concentration needed for Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
1 Antwort-
This is a 1:1 mixture of the DME and F-12. The optimal carbon dioxide required for DME is 10% and for F-12 is 5%. Since this medium is a mixture, the optimal carbon dioxide concentration is typically 5% to 8%.
Hilfreich?
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What is the storage temperature of Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
1 Antwort-
We recommend storing Product No. D8437 at 2-8°C.
Hilfreich?
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What is Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
1 Antwort-
This product is a 1:1 ratio mixture of Dulbecco’s Modified Eagle’s Medium (DME) high glucose and Nutrient Mixture F-12 Ham (F-12).
Hilfreich?
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