Bei der Karyotypisierung und in der Zellzyklusforschung ist es oft wünschenswert, die Ausbeute an mitotischen Zellen in einer bestimmten Phase des Zellzyklus zu erhöhen. Hierzu gibt es mehrere Möglichkeiten; am beliebtesten ist die Verwendung eines Zellzyklus-Synchronisierungsmittels wie etwa Demecolcin. Demecolcin arretiert Zellen in der Metaphase und hat dabei keine merklichen Auswirkungen auf die biochemischen Ereignisse in mitotischen Zellen oder in Zellen, die in der G1- und der S-Phase synchronisiert wurden. Weiße Blutkörperchen werden häufig mit Demecolcin behandelt, um sie in der Metaphase anzuhalten.
Sonstige Hinweise
Binds tubulin hence interfering with microtubule-dependent cell function[1]
European journal of biochemistry, 142(3), 577-581 (1984-08-01)
Colcemid binds tubulin rapidly and reversibly in contrast to colchicine which binds tubulin relatively slowly and essentially irreversibly. At 37 degrees C the association rate constant for colcemid binding is 1.88 X 10(6) M-1 h-1, about 10 times higher than
The Journal of biological chemistry, 285(42), 32242-32250 (2010-08-11)
Drugs that target microtubules are thought to inhibit cell division and cell migration by suppressing dynamic instability, a "search and capture" behavior that allows microtubules to probe their environment. Here, we report that subtoxic drug concentrations are sufficient to inhibit
Prolonged in vitro culture of human embryonic stem (hES) cells can result in chromosomal abnormalities believed to confer a selective advantage. This potential occurrence has crucial implications for the appropriate use of hES cells for research and therapeutic purposes. In
BioEssays : news and reviews in molecular, cellular and developmental biology, 34(9), 760-770 (2012-06-19)
Recent studies indicate that mammalian chromosomes contain discrete cis-acting loci that control replication timing, mitotic condensation, and stability of entire chromosomes. Disruption of the large non-coding RNA gene ASAR6 results in late replication, an under-condensed appearance during mitosis, and structural
Reproduction in domestic animals = Zuchthygiene, 46(4), 699-704 (2010-12-08)
The present investigation was carried out to find an efficient chemically assisted procedure for enucleation of goat oocytes related to handmade cloning (HMC) technique. After 22-h in vitro maturation, oocytes were incubated with 0.5 μg/ml demecolcine for 2 h. Cumulus
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