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XNAPG

Sigma-Aldrich

SYBR® Green Extract-N-Amp™ Plant PCR Kit

sufficient for 100 preparations

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About This Item

UNSPSC Code:
41106303
NACRES:
NA.55

usage

sufficient for 100 preparations
 sufficient for 100 reactions

Quality Level

200

feature

dNTPs included
hotstart

technique(s)

qPCR: suitable

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

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General description

SYBR® Green Extract-N-Amp™ Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.

Features and Benefits

  • Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • No loading buffers or tracking dyes required for gel analysis
  • SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
  • SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
  • Compatible with high-throughput requirements for genetic analysis of plants
  • No long enzymatic digestions
  • Extract stable at 4°C for at least 6 months

Other Notes

The protocol uses a 20 μl PCR reaction.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list
  • E7526Extraction SolutionBezpečností list
  • D5688Dilution SolutionBezpečností list
  • S4320SYBR® Green Extract-N-Amp™ PCR ReadyMix™Bezpečností list

related product

Č. produktu
Popis
Stanovení ceny

W1754

Water, PCR Reagent

P6097

Precast Agarose Gels, 24 wells (2 × 12-well portrait orientation)

XNAP

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

T4415

Tris-Borate-EDTA buffer, BioReagent, suitable for electrophoresis, 10× concentrate

T9525

Tris-Borate-EDTA buffer, working solution

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

P9577

PCR 50 - 2,000 bp Marker, for electrophoresis of PCR fragments

XNAPE

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications

XNAP2

Extract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAR

Extract-N-Amp™ Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPR

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPS

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

R4775

REDExtract-N-Amp™ PCR ReadyMix™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

D5688

Dilution Solution, Extract-N-Amp Plant Dilution Solution

S4320

SYBR® Green Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
SLCR5690
SLCM2893
SLCJ5970
SLCC2950
SLBN7468V

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