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Sigma-Aldrich

Extract-N-Amp™ Plant PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

Synonyma:

Plant direct PCR kit

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About This Item

UNSPSC Code:
41106303
NACRES:
NA.55

usage

sufficient for 1000 amplifications
 sufficient for 1000 extractions

feature

hotstart

technique(s)

PCR: suitable

color

colorless

shipped in

wet ice

storage temp.

−20°C

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General description

The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.

Application

Extract-N-Amp™ Plant PCR Kit has been used for DNA extraction from fresh mushrooms.
Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

Features and Benefits

  • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
  • A specially formulated PCR ReadyMix is provided for use with the extracted DNA
  • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
  • Suitable for high-throughput plant genetic analysis.
  • Lengthy enzymatic digestions are not required, saving time and effort.
  • The extracted DNA remains stable at 4 °C for atleast 6 months.

Components

Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Related product

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Stanovení ceny

XNAP

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAPE

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications

XNAP2

Extract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAPR

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPS

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

R4775

REDExtract-N-Amp™ PCR ReadyMix™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

D5688

Dilution Solution, Extract-N-Amp Plant Dilution Solution

XNAPG

SYBR® Green Extract-N-Amp™ Plant PCR Kit, sufficient for 100 preparations

S4320

SYBR® Green Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Navštívit knihovnu dokumentů

Alison Heffer et al.
EvoDevo, 2(1), 7-7 (2011-03-03)
Tremendous progress has been made in the field of evo-devo through comparisons of related genes from diverse taxa. While the vast number of species in nature precludes a complete analysis of the molecular evolution of even one single gene family
Rapid and reliable high-throughput methods of DNA extraction for use in barcoding and molecular systematics of mushrooms
Dentinger BTM, et al.
Molecular Ecology Resources, 10(4), 628-633 (2010)
Antoine Lf Gady et al.
Plant methods, 5, 13-13 (2009-10-09)
The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as
M Riahi et al.
Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)
Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with
Matthew E Smith et al.
PloS one, 8(1), e55160-e55160 (2013-02-06)
Ectomycorrhizal (ECM) plants and fungi can be diverse and abundant in certain tropical ecosystems. For example, the primarily paleotropical ECM plant family Dipterocarpaceae is one of the most speciose and ecologically important tree families in Southeast Asia. Pakaraimaea dipterocarpacea is
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Sortimentní položky

Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Příručka k produktu Extract-N-Amp™

Soupravy Extract-N-Amp™ PCR jsou prvním integrovaným procesem extrakce a amplifikace na světě pro rychlé testy krve, tkání nebo rostlin.

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

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Protokoly

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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