Přejít k obsahu
Merck
Všechny fotografie(1)

Key Documents

S8015

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Všechny fotografie(1)

About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

grade

for molecular biology

form

powder blend

pH

6.9-7.1 (20-25 °C, 20 ×)

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

General description

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

Application

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

Features and Benefits

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Components

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

related product

Č. produktu
Popis
Stanovení ceny

D9156

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

D0428

1 kb DNA Ladder, for DNA electrophoresis

H7033

PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

T9285

Tris-EDTA buffer solution, 100X concentrate for Northern and Southern blotting

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

B6185

Bionic™ Buffer, 10X Concentrate, for Northern and Southern blotting, solution

GERPN3005

ECL™ Direct Nucleic Acid, Cytiva RPN3005, pack of 1 ea

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Zákazníci si také prohlíželi

Slide 1 of 4

1 of 4

Potassium hydroxide BioXtra, ≥85% KOH basis

Sigma-Aldrich

P5958

Potassium hydroxide

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide ≥97.0% (T)

Sigma-Aldrich

39391

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide

Sodium cyanoborohydride reagent grade, 95%

Sigma-Aldrich

156159

Sodium cyanoborohydride

Sodium bicarbonate powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

S5761

Sodium bicarbonate

A J Bogers et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 2(3), 160-166 (1988-01-01)
To identify risk factors for hospital mortality after postinfarction left ventricular aneurysmectomy (PILVA), 83 consecutive cases of PILVA were studied in a retrospective analysis. Hospital mortality was 14.4%. Most of the factors correlated with hospital mortality were associated with poor
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how

Protokoly

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.