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Key Documents

P7059

Sigma-Aldrich

Phosphate Buffered Saline

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

Synonyma:

Phosphate buffered saline, PBS

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About This Item

MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate Buffered Saline, 10× PBS for Western blots and IP

grade

for molecular biology

sterility

0.2 μm filtered

form

liquid

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

pH

7.2-7.6(1x solution)

storage temp.

room temp

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General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Components

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate

Preparation Note

Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Sortimentní položky

Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.

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