There is no specific information available about storing the enzyme at -80°C once it has been reconstituted.
P5147
Protease from Streptomyces griseus
Type XIV, ≥3.5 units/mg solid, powder
Synonyma:
Actinase E, Pronase E
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Vybrat velikost
100 MG
1 490,00 Kč
1 G
4 890,00 Kč
5 G
15 500,00 Kč
1 490,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Vyžádat hromadnou objednávku
Vybrat velikost
Změnit zobrazení
100 MG
1 490,00 Kč
1 G
4 890,00 Kč
5 G
15 500,00 Kč
About This Item
Číslo CAS:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
1 490,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Vyžádat hromadnou objednávku
Doporučené produkty
biological source
Streptomyces griseus
Quality Level
type
Type XIV
form
powder
specific activity
≥3.5 units/mg solid
solubility
10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble 0.2 mg/mL at 37 °C, clear, colorless to tan
shipped in
wet ice
storage temp.
−20°C
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Související kategorie
Specificity
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
application
Protease from Streptomyces griseus has been used:
- for the digestion of nucleus pulposus (NP) tissue[1]
- in the catalysis of asymmetric one pot Mannich reaction[2]
- for deproteinization of starch[3]
- digestion of brain slices for the cell dissociation[4]
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Streptomyces griseus has been used in crystallographic and kinetic investigations of the covalent complex formed by tetrapeptide aldehydes and serine proteases[5].
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
The enzyme from Sigma has been used for the digestion and analysis of antithrombin-heparin complexes.[6] It has also been used for the isolation of enzyme-resistant starch.[7]
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease from Streptomyces griseus is a mixture of at least three proteolytic activities including an extracellular serine protease. Serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
This product is a mixture of at least three caseinolytic activities and one aminopeptidase activity.[8] The caseinolytic enzymes were named as Streptomyces griseus Protease A, Streptomyces griseus Protease B and Streptomyces griseus Trypsin. This product may be used when extensive or complete degradation of protein is required. This protease mixture is highly nonspecific and can digest casein to the extent of >70% as mono-amino acids.
Features and Benefits
- highly stable in pH range of 5.0 to 9.0, with peak activity at pH 8.8
- compatible with many DNA and RNA isolation buffers
- broad substrate specificity
Quality
Contains calcium acetate.
Physical properties
Completely inactivated by heating above 80 °C for 15-20 minutes.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Preparation Note
Collected from culture broth of S. griseus.
related product
Č. produktu
Popis
Stanovení ceny
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Osvědčení o analýze (COA)
Lot/Batch Number
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Zákazníci si také prohlíželi
Simone Valentin Hansen et al.
PloS one, 13(10), e0205461-e0205461 (2018-10-12)
Drosophila DNA replication-related element binding factor (DREF) is a transcription regulatory factor that binds the promoters of many genes involved in replication and cell proliferation and is required for normal cell cycle progression. Human DREF/zinc finger BED domain-containing protein 1
Enzyme-resistant starch. I. Quantitative and qualitative influence of incubation time and temperature of autoclaved starch on resistant starch formation
Eerlingen RC, et al.
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Changes of AMPA receptor properties in the neocortex and hippocampus following pilocarpine-induced status epilepticus in rats
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Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells
Purmessur D, et al.
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An improved fractionation system for pronase on CM-sephadex.
J Jurásek et al.
Canadian journal of biochemistry, 49(11), 1195-1201 (1971-11-01)
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Is it possible to store P5147 at -80°C after reconstitution?
1 answer-
Helpful?
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Under what conditions can Product P5147, Protease from Streptomyces griseus, be used to digest protein?
1 answer-
For DNA isolation, Pronase E is usually prepared as a stock solution, and prior to storage at -20°C, the solution is first heated to 56°C for about 15 minutes, then incubated at 37°C for 1 hour. This encourages self-digestion, to eliminate DNAse and RNAse contamination. The enzyme is added to a DNA sample (in the presence of 0.5-1%(w/v) SDS to disrupt DNA protein interactions) typically at 250-500 μg protein/mL, then incubated at 37°C for 1-4 hours. For protein hydrolysis, dissolve about 0.2 μmol of protein in 0.2 mL of 50 mM ammonium bicarbonate buffer at pH 8 (or phosphate buffer pH 7). Add Pronase to 1% (w/w) and incubate at 37°C for 24 hours. It may be necessary to add aminopeptidase M at 4% (w/w) and incubate at 37°C for another 18 hours.
Helpful?
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Is Product P5147, Protease from Streptomyces griseus, also described as "Pronase"?
1 answer-
"Pronase E" is the name given to a group of proteolytic enzymes produced by Streptomyces griseus K-1. At least 10 proteases are in the mixture, five serine-type proteases, two zinc endopeptidases, two zinc leucine aminopeptidases, and one zinc carboxypeptidase.
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Does Product P5147, Protease from Streptomyces griseus, contain calcium acetate?
1 answer-
This product contains calcium acetate buffer salt. The amount of calcium acetate present will vary for each lot number and has been observed to range from 2 - 30% (w/w).
Helpful?
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How should Product P5147, Protease from Streptomyces griseus, be reconstituted and how should a stock solution be prepared?
1 answer-
This product can be dissolved in 0.01 M sodium acetate with 0.005 M calcium acetate at pH 7.5 at 37°C; at 0.2 mg/mL, this yields a clear solution which ranges from colorless to light tan. Calcium ion is recommended for protection from autolysis. The activity of a dilute enzyme solution containing 0.01 to 0.1 M calcium ion was stable over 24 hours at neutral pH at 2-8°C. Pronase E is stable at 4°C for at least six months. Stock solutions of 5 to 20 mg/mL in water are usually stored at -20°C.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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