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MITOISO1

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Synonyma:

Mitochondria Purification Kit

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

EC Number:
232-650-8
UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

200

usage

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

shelf life

2 yr

technique(s)

fractionation: suitable

shipped in

dry ice

storage temp.

−20°C

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Application

Mitochondria Isolation Kit has been used:
  • for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
  • for the isolation of mitochondria from glomeruli and heart
  • for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Features and Benefits

  • Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
  • Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
  • Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
  • Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidBezpečností list

related product

Č. produktu
Popis
Stanovení ceny

CYTOCOX1

Cytochrome c Oxidase Assay Kit, sufficient for 100 tests, soluble and membrane bound mitochondria

CS0720

Citrate Synthase Assay Kit, 1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

MITOISO2

Mitochondria Isolation Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

CS0760

Isolated Mitochondria Staining Kit, 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

MITOISO3

Yeast Mitochondria Isolation Kit, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
0000284757
0000284757
0000222575
0000222575
0000137714

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Sortimentní položky

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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