E0887
Esterase from rabbit liver
lyophilized powder, ≥30 units/mg protein
Synonyma:
Carboxyl esterase, Carboxylic-ester hydrolase
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
Doporučené produkty
form
lyophilized powder
specific activity
≥30 units/mg protein
mol wt
60 kDa
composition
Protein, ≥85% biuret
storage temp.
−20°C
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General description
Esterase belongs to the C-family of carboxylesterases. It is a glycoprotein, mainly present in the endoplasmic reticulum of hepatocytes.
Application
Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on expression and activity of a lipoprotein-associated phospholipase A(2).
Esterase from rabbit liver has been used:
- in comparative biophysical studies with bovine bile-salt-activated lipase in the assays with vinyl acetate, triacylglycerols, p-nitrophenyl esters and serine enzyme inhibitors
- in the catalysis and kinetic profiling studies of peptidic compounds
- in in vitro stability assays of prodrug compounds (BMS-292655 and BMS300043)
- in the hydrolysis of oleuropein aglycone (Ole-AG)
The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.
Biochem/physiol Actions
Esterase enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.
Esterase enzyme has broad substrate specificity. Esterase from rabbit liver has been used to investigate the toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used for the hydrolysis of simvastatin.
Unit Definition
One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.
Physical form
Lyophilized powder containing Tris buffer salts
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protokoly
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
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