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E2884

Sigma-Aldrich

Esterase from porcine liver

ammonium sulfate suspension, ≥150 units/mg protein (biuret)

Synonyma:

Carboxyl esterase, Carboxylic-ester hydrolase, PLE

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About This Item

Číslo CAS:
9016-18-6
Číslo enzymu podle klasifikace EK:
3.1.1.1 (BRENDA, IUBMB)
MDL number:
MFCD00131030
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Porcine liver

form

ammonium sulfate suspension

specific activity

≥150 units/mg protein (biuret)

mol wt

168 kDa

storage temp.

2-8°C

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General description

Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.

Application

Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.
The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Biochem/physiol Actions

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.
Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.
Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 8

Substrate

Č. produktu
Popis
Stanovení ceny

N8000

1-Naphthyl butyrate, esterase substrate

B4377

5-Bromo-4-chloro-3-indolyl acetate, esterase substrate

I3500

Indoxyl acetate, ≥95% (GC)

N8130

4-Nitrophenyl acetate, esterase substrate

N0758

Naphthol AS-D chloroacetate, esterase substrate

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Osvědčení o analýze (COA)

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Karl Vollmann et al.
Molecules (Basel, Switzerland), 13(2), 348-359 (2008-02-29)
The compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4) was synthesized as an amino acid ester prodrug of the adenosine A2A receptor antagonist MSX-2. It was found to be stable in artificial gastric acid, but readily cleaved by pig liver esterase.
Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells
Watkins RW, et al.
Organic & Biomolecular Chemistry, 7(19), 3969-3975 (2019)
B Wang et al.
Methods in molecular medicine, 23, 71-85 (1999-01-01)
With the discovery of an increasing number of biologically active peptides and peptide mimetics (1-3), there is a pressing need for the development of strategies to deliver these biologically active compounds to the desired site of action. The preceding two
F Dorandeu et al.
Toxicology, 248(2-3), 151-157 (2008-05-03)
Organophosphorus chemical warfare agents (nerve agents) are to be feared in military operations as well as in terrorist attacks. Among them, VX (O-ethyl-S-[2-(diisopropylamino)ethyl] methylphosphonothioate) is a low volatility liquid that represents a percutaneous as well as an inhalation hazard if
Robert L Wilensky et al.
Nature medicine, 14(10), 1059-1066 (2008-09-23)
Increased lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) activity is associated with increased risk of cardiac events, but it is not known whether Lp-PLA(2) is a causative agent. Here we show that selective inhibition of Lp-PLA(2) with darapladib reduced development of advanced coronary
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Sortimentní položky

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protokoly

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

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