BHK21-InVitrus

5062302, hamster kidney, Fibroblast

• UNSPSC Code: 41106514

Vlastnosti

• Název produktu: BHK21-InVitrus, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 05062302
• biological source: hamster kidney
• packaging: tube of 5 μg 05062302-DNA-5UG; pkg of vial of cells 05062302-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Hamster Syrian kidney, serum-free

Cell Line Description

BHK21CL13-2P cell line (Sigma Catalogue number. 84111301) adapted to grow in the chemically defined protein- and peptide-free InVitrus^™ medium.

Culture Medium

InVitrus^™ medium + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use InVitrus medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.

Subculture Routine

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Legal Information

InVitrus is a trademark of Cell Culture Technologies