MDCK Cell Line canine

84121903, canine kidney, Epithelial

• Synonyma: MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• UNSPSC Code: 41106514
• eCl@ss: 32190102

Vlastnosti

• product name: MDCK Cell Line canine, 84121903
• biological source: canine kidney
• form: cell suspension
• growth mode: Adherent
• karyotype: 2n = 78
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Canine Cocker Spaniel kidney

Cell Line Description

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.

Application

Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Other Notes

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