MDBK Cell Line bovine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90050801, bovine kidney, Epithelial-like

• Synonyma: MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells
• eCl@ss: 32190102
• UNSPSC Code: 41106514
• Biological source: bovine kidney
• Growth mode: Adherent
• Karyotype: Modal no. 51; hypodiploid
• Morphology: Epithelial-like

Vlastnosti

• biological source: bovine kidney
• packaging: tube of 5 μg 90050801-DNA-5UG, pkg of vial of cells 90050801-1VL
• growth mode: Adherent
• karyotype: Modal no. 51; hypodiploid
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.

Biochem/physiol Actions

Bovine kidney

Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.