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A7437

Sigma-Aldrich

Acetate Kinase from Escherichia coli

lyophilized powder, ≥150 units/mg protein (biuret)

Synonyma:

ATP:Acetate phosphotransferase

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About This Item

Číslo CAS:
9027-42-3
Číslo enzymu podle klasifikace EK:
2.7.2.1 (BRENDA, IUBMB)
MDL number:
MFCD00130330
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥150 units/mg protein (biuret)

composition

Protein, 5.0-20.0%

UniProt accession no.

P0A6A3

foreign activity

adenosine triphosphatase and NADH oxidase ≤0.01%

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... ackA(946775)

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General description

Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily. It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.

Application

Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation .

Biochem/physiol Actions

Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.
Involved in the metabolism of propanoate, pyruvate and taurine

Unit Definition

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Navštívit knihovnu dokumentů

Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases
Buss KA, et al.
Journal of Bacteriology, 183(2), 680-686 (2001)
S C Olson et al.
The Journal of biological chemistry, 266(26), 17236-17242 (1991-09-15)
Receptor-linked activation of phospholipase D has been demonstrated recently in a variety of intact cell types including granulocytes, but little is known about the enzyme, its cofactor requirements, and regulation. Using [3H]alkyllysophosphatidylcholine to prelable an endogenous phosphatidylcholine substrate pool in
Influence of residual ethanol concentration on the growth of Gluconacetobacter xylinus I 2281
Kornmann H, et al.
Applied Microbiology and Biotechnology, 62(2-3), 168-173 (2003)
Hélène Scornec et al.
Frontiers in microbiology, 11, 156-156 (2020-03-21)
The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (cwaR), LSEI_0220 (cwaS), and LSEI_0221 (ldcA), has been previously identified as required for the establishment of Lactobacillus paracasei in the
Andrea Gorrell et al.
The Journal of biological chemistry, 280(11), 10731-10742 (2005-01-14)
Acetate kinase catalyzes transfer of the gamma-phosphate of ATP to acetate. The only crystal structure reported for acetate kinase is the homodimeric enzyme from Methanosarcina thermophila containing ADP and sulfate in the active site (Buss, K. A., Cooper, D. C.
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