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A3983

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, essentially γ-globulin free, ≥98% (agarose gel electrophoresis)

Synonyma:

Albumin bovine serum, BSA, Bovine albumin

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About This Item

Číslo CAS:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.25

biological source

bovine

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

New Zealand origin

impurities

essentially globulin free

loss

≤5%

pH

7

solubility

water: soluble, clear to slightly hazy (1 g + 25 mL)

UniProt accession no.

P02769

foreign activity

Protease free

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Související kategorie

General description

Bovine Serum Albumin has three homologous domains (I, II, and III) that are connected by disulfide bonds. BSA shares structural homology with human serum albumin (HSA).

Application

Bovine Serum Albumin has been used:
  • as a component of blocking solution used for fixing Drosophila embryos in immunostaining
  • to block 96-deep-well plates in phage immunoprecipitation studies
  • as a standard solution in fluorescence spectroscopy studies

Biochem/physiol Actions

Bovine Serum Albumin (BSA) displays intrinsic fluorescence due to the presence of two tryptophan residues. It is widely used in ligand interaction studies and in the delivery of drugs and antibodies. BSA is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Derived from New Zealand source serum
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Osvědčení o analýze (COA)

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Navštívit knihovnu dokumentů

Evaluation of biophysical interaction between newly synthesized pyrazoline pyridazine derivative and bovine serum albumin by spectroscopic and molecular docking studies
Al-Mehizia AA, et al.
Journal of Spectroscopy (New York, NY, United States), 2019 (2019)
Yifan Wang et al.
Drug design, development and therapy, 15, 1531-1547 (2021-04-23)
siRNA brings hope for cancer therapy. However, there are many obstacles for application of siRNA in clinical. Because of the excellent biocompatibility, non-toxicity and non-immunogenicity of bovine serum albumin (BSA), BSA-based nanoparticles have been widely designed as a drug carrier
Helen Weavers et al.
Current biology : CB, 29(22), 3851-3862 (2019-11-02)
In healthy individuals, injured tissues rapidly repair themselves following damage. Within a healing skin wound, recruited inflammatory cells release a multitude of bacteriocidal factors, including reactive oxygen species (ROS), to eliminate invading pathogens. Paradoxically, while these highly reactive ROS confer
Ceri-Anne E Suurmond et al.
Advanced healthcare materials, 8(24), e1901379-e1901379 (2019-11-21)
The liver has a complex and unique microenvironment with multiple cell-cell interactions and internal vascular networks. Although nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease with multiple phases, no proper model could fully recapitulate the in
Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
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