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Key Documents

56822

Sigma-Aldrich

Immersion oil

for microscopy

Synonyma:

immersion medium for light microscopy

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About This Item

MDL number:
MFCD00131369
UNSPSC Code:
12171500
NACRES:
NA.47

biological source

synthetic

Quality Level

100

grade

for microscopy

form

liquid

refractive index

n20/D 1.516

viscosity

100-120 mPa.s(20 °C)

density

1.025 g/mL at 20 °C

application(s)

hematology
histology

storage temp.

room temp

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General description

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power.

Application

Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.

Principle

Immersion oil is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.

pictograms

Environment
GHS09

signalword

Warning

hcodes

H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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