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198161

Sigma-Aldrich

Toluidine Blue O

certified by the Biological Stain Commission

Synonyma:

Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride

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About This Item

Empirický vzorec (Hillův zápis):
C15H16ClN3S
Číslo CAS:
92-31-9
Molekulová hmotnost:
305.83
Colour Index Number:
52040
EC Number:
202-146-2
MDL number:
MFCD00011934
UNSPSC Code:
12171500
PubChem Substance ID:
24851865
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, 80%

color

dark green

λmax

626 nm

ε (extinction coefficient)

≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

InChI key

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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Související kategorie

General description

A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.

Biochem/physiol Actions

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.

Suitability

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
SHBQ4530
SHBR8115
SHBP9405
SHBQ3835
SHBP2785

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Potřebujete-li konkrétní verzi, můžete vyhledat daný certifikát podle čísla dávky nebo čísla šarže.

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The Effect of Variable Energy Input from a Novel Light Source on the Photoactivated Bactericidal Action of Toluidine Blue O on Streptococcus mutans
Williams J A
Caries Research, 37, 190?193-190?193 (2003)
Anaīs Noblanc et al.
Antioxidants (Basel, Switzerland), 9(2) (2020-02-15)
There is growing evidence that the quality of spermatozoa decreases with age and that children of older fathers have a higher incidence of birth defects and genetic mutations. The free radical theory of aging proposes that changes with aging are
Polychromatic staining of plant cell walls by toluidine blue O
Brien T P O
Protoplasma, 59(2), 368- 373 (1964)
Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode.
Zanin IC
European Journal of Oral Sciences, 114(1), 64-69 (2006)
Catherine White
Best practice & research. Clinical obstetrics & gynaecology, 27(1), 113-130 (2012-12-12)
The examination of the rape victim should focus on the therapeutic, forensic and psychological needs of the individual patient. One aspect will be an examination for ano-genital injuries. From a medical perspective, they tend to be minor and require little
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