Yes, in addition to biological stain applications, Methylene Blue is also commonly used as a redox indicator. Oxidized solutions will appear blue in color and will turn colorless when mixed with a reducing agent. A reduced or clear solution will turn blue when exposed to an oxidizing agent. Methylene blue is considered a basic dye, but not commonly used as an pH indicator. Methyl Blue, which is a chemically different compound, is more commonly used as a pH indicator. Please see the link below to review this compound:
https://www.sigmaaldrich.com/product/sial/95290
M9140
Methylene blue
Dye content, ≥82%, certified by the Biological Stain Commission, powder
Synonyma:
Basic Blue 9, 3,7-bis(Dimethylamino)phenazathionium chloride, Tetramethylthionine chloride
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
25 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 840,00 Kč |
100 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 9 430,00 Kč |
O této položce
Empirický vzorec (Hillův zápis):
C16H18ClN3S · xH2O
Číslo CAS:
Molekulová hmotnost:
319.85 (anhydrous basis)
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
200-515-2
MDL number:
Colour Index Number:
52015
Beilstein/REAXYS Number:
3599847
2 840,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Methylene blue, certified by the BSC
agency
certified by the BSC
Quality Segment
form
powder
composition
Dye content, ≥82%
technique(s)
microbe id | staining: suitable
color
dark green
solubility
H2O: 4mg/4ml
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CN(C)C1=CC=C2C(SC(C3=N2)=CC(C=C3)=[N+](C)C)=C1.[H]O[H].[Cl-]
InChI
1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
InChI key
WQVSELLRAGBDLX-UHFFFAOYSA-M
Gene Information
human ... CYP1A2(1544), GSR(2936)
General description
Methylene blue is a compound that occurs as crystals or crystalline powder, having a bronze-like luster. It is soluble in water, chloroform and partially soluble in alcohol. It is a photoactive dye, which is a member of phenothiazine family.
Application
Methylene blue has been used:
- to stain vaginal cells
- for the preparation of protein aqueous ink
- to evaluate cell survival by staining
Certified for use in Mallory′s phloxine-methylene blue stain; Levine & Black stain for bacteria in milk; Wright stain for blood smears.
Biochem/physiol Actions
Methylene blue is used as a bacteriologic stain or dye and indicator. It mediates the inactivation of enveloped viruses in plasma, when exposed to visible light. It is an inhibitor of nitric oxide synthase and guanylate cyclase. It also plays a key role in medicine as an antimalarial agent, reducing agent in methemoglobinemia and inducer of apoptosis in cancer cells.
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Tato položka | |||
|---|---|---|---|
| description certified by the BSC | description certified reference material, pharmaceutical secondary standard | description United States Pharmacopeia (USP) Reference Standard | description ≥95% (calc. to the dried substance) |
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - | technique(s) - |
| color dark green | color - | color - | color very dark green |
| form powder | form - | form - | form powder |
| solubility H2O: 4mg/4ml | solubility - | solubility - | solubility ethanol: insoluble ≤1% |
| application(s) diagnostic assay manufacturing | application(s) pharmaceutical | application(s) pharmaceutical (small molecule) | application(s) diagnostic assay manufacturing |
| storage temp. room temp | storage temp. 2-8°C | storage temp. - | storage temp. room temp |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Skladovací třída
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Is this ingredient suitable for use as a redox indicator? What is the main difference compered to that used as an indicator?
1 answer-
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Which is the granulometry of this powder?
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The quality control testing only requires that the methylene blue powder appear as a powder. There is no requirement to define the powder as either coarse, medium or fine powder.
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Are various powdered methylene blues suitable for marking brain regions in live mice? There are issues visualizing it after staining, suspected due to outdated methylene blue. Assistance is required in choosing the right type for preparation for identifying specific brain regions.
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There is no mention of which methylene blue has been problematic. Biological dyes usually lack expiration dates. The recommended catalog number is M9140, which is certified by the Biological Stain Commission (BSC) for staining nuclei blue using paraffin sections and for staining bacteria in milk. Other applications of Methylene Blue include staining white blood cells in Wright Stain, oversight staining, supra vital staining, and staining nerves, nerve fibers, neuronal processes, live non-neuronal cells, tumors, and as a surgical marker.Fresh and aged dyes can yield different reactions. Effective solutions may need fresh preparation. Some dyes have set expiration dates due to deterioration. While using two blue dyes is unusual; thionin is commonly used for Nissl staining.
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Is there any information available on ammonium molybdate's role in fixing methylene blue dye?
1 answer-
There is no information available regarding the use of ammonium molybdate for mixing methylene blue staining.
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Is there any testing conducted for lead and other heavy metals in any of the available methylene blue products?
1 answer-
Medicinal grade and food grade dyes are likely to undergo testing for lead and other heavy metals. It is unlikely that Methylene Blue is offered as a food grade dye. M9140 is not subjected to testing for lead and other heavy metals because it is not intended for human or veterinary use.
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How is dye content determined for Methylene blue, Product M9140?
1 answer-
Dye content is determined spectrophotometrically by noting the dye's absorbance peak, which is typically near 665 nm, or by calculating the ratio of the absorbances at 650 nm over 680 nm. (The dye exhibits smaller peaks at approximately 292 and 246 nm).
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What is the solubility of Methylene blue, Product M9140?
1 answer-
The product is soluble in water at 50 mg/mL, EGME at 60 mg/mL and ethanol at 70 mg/mL. EGME is an abbreviation for ethylene glycol monomethyl ether, also known as 2-methoxyethanol or methyl cellosolve.
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How can Methylene blue, Product M9140, be used?
1 answer-
Probably its most important application is as an in vitro diagnostic bacteriology, cytology, hematology, and histology stain. Methylene Blue serves as a precursor to the Methylene Azures and as a component of the various Ramanowsky-type blood stains (Wright, Leishman, Giemsa and others). While pure Methylene Blue is not a good nuclear stain, it readily undergoes oxidative demethylation to form the various Methylene Azures, which are good nuclear stains.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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