8.51072

6-Carboxyfluorescein

Novabiochem^®

• Synonyma: 6-Carboxyfluorescein, 6-FAM
• Empirický vzorec (Hillův zápis): C₂₁H₁₂O₇
• Číslo CAS: 3301-79-9
• Molekulová hmotnost: 376.32
• MDL number: MFCD00036873
• UNSPSC Code: 12352005
• NACRES: NA.22

Vlastnosti

• Quality Level: 200
• product line: Novabiochem^®
• assay: ≥95% (TLC); ≥98.0% (HPLC)
• form: powder
• reaction suitability: reagent type: fluorescent-labelling reagent
• manufacturer/tradename: Novabiochem^®
• application(s): peptide synthesis
• storage temp.: 2-8°C
• SMILES string: O1C3(c4c(cc(cc4)O)Oc5c3ccc(c5)O)c2c(ccc(c2)C(=O)O)C1=O
• InChI: 1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI key: BZTDTCNHAFUJOG-UHFFFAOYSA-N

Popis

General description

This high purity fluorescent dye (λ_em494 nm, λ_ex 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or protected by tritylation prior to acylation or hydrazine treatment ^[1].

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Linkage

Replaces: 01-63-0113

Analysis Note

Color (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 95 %
Assay (HPLC, area%): ≥ 98.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable