86826

5-Carboxyfluorescein

BioReagent, suitable for fluorescence, ≥95% (HPLC)

• Synonyma: 5-FAM
• Empirický vzorec (Hillův zápis): C₂₁H₁₂O₇
• Číslo CAS: 76823-03-5
• Molekulová hmotnost: 376.32
• Beilstein/REAXYS Number: 57037
• MDL number: MFCD00036874
• UNSPSC Code: 12171500
• PubChem Substance ID: 57653195
• NACRES: NA.32

Vlastnosti

• product line: BioReagent
• Quality Level: 100
• assay: ≥95% (HPLC)
• form: powder
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble
• fluorescence: λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0
• suitability: suitable for fluorescence
• SMILES string: OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI key: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Popis

General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)