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GE17-0405-03

HiTrap® Protein G High Performance

Cytiva 17-0405-03, pack of 5 × 5 mL

Sinónimos:

HP columns, Protein G Sepharose column

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

envase

pack of 5 × 5 mL

fabricante / nombre comercial

Cytiva 17-0405-03

condiciones de almacenamiento

(20% Ehtanol)
(20% Ethanol)

Parámetros

42 psi

lecho tamaño

16 mm × 25 mm

lecho volumen

5 mL

columna D.I.

16 mm

tamaño

tam. prom. pieza

34 μm

cleaning

2-9

Intervalo de trabajo

3-9

capacidad

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
125 mg binding capacity(human IgG/column)

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Características y beneficios

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


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Artículos

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

Ver todo

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