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Key Documents

S0677

Sigma-Aldrich

Estreptavidina from Streptomyces avidinii

recombinant, expressed in E. coli, lyophilized powder

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About This Item

Número de CAS:
9013-20-1
MDL number:
MFCD00082035
UNSPSC Code:
12352203
NACRES:
NA.46

recombinant

expressed in E. coli

form

lyophilized powder

capacity

4 mol/mol binding capacity (Biotin)

storage temp.

−20°C

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General description

Streptavidin is a biotin-binding protein that is obtained from Streptomyces avidinii. It is resistant to extreme temperature, pH, detergents and proteolytic enzymes. Streptavidin has a molecular weight of 60kDa and exists as a homotetramer.

Application

Streptavidin from Streptomyces avidinii has been used-
  • for blocking of the AFM (atomic force microscopy) tip, during the measurement of unbinding forces in the avidin-biotin system
  • for coupling of anti-HA (hemagglutinin) antibodies during cell free synthesis and assembly of proteins on a biochip
  • for the coating of the gelatin flow phantoms to eventually determine the feasibility of IVUS (intravascular ultrasound) transducer for (biotinylated) microbubble-based drug delivery
  • to immobilize proteins and oligonucleotides

Biochem/physiol Actions

Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization and affinity chromatography.

Unit Definition

One unit will bind 1.0 μg biotin.

Physical form

Lyophilized from 5 mM potassium phosphate buffer

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Cell-free protein synthesis and assembly on a biochip.
Heyman Y et al
Nature Nanotechnology, 7(6), 374-378 (2012)
Polymer Nanoparticles
Progress in Molecular Biology and Translational Science, 104 (2011)
Determination of binding curves via protein micropatterning in vitro and in living cells
Stefan S
Cytometry (2012)
Nicola Ilk et al.
Applied and environmental microbiology, 68(7), 3251-3260 (2002-06-29)
The nucleotide sequence encoding the crystalline bacterial cell surface (S-layer) protein SbpA of Bacillus sphaericus CCM 2177 was determined by a PCR-based technique using four overlapping fragments. The entire sbpA sequence indicated one open reading frame of 3,804 bp encoding
Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation
Nuttada Panpradist
PLoS ONE (2016)
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