The extinction coefficient for the product in question is 2.8 (E0.1%).
SAE0094
SAvPhire™ Monomeric Streptavidin
recombinant, expressed in E. coli, High Affinity
Sinónimos:
mSA3
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
recombinante
expressed in E. coli
Formulario
lyophilized powder
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Acciones bioquímicas o fisiológicas
SAvPhire™ Monomeric Streptavidin is a variant of the biotin-binding streptavidin, which naturally exists as a tetramer. Many monomeric streptavidin proteins have rapid dissociation kinetics, which prevent their use in stable labeling applications. However, SAvphire™ Monomeric Streptavidin has been engineered to have a dissociation rate comparable to that of tetrameric streptavidin.
The size and monovalency of this product provide advantages over tetrameric streptavidin in applications such as functional cell surface labeling and atomic force spectroscopy. These advantages include:
This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.
- Minimizing distribution or dynamics in the probing of biotinylated targets
- Reducing risk of aggregation, precipitation, and cross-linking in pre-incubation steps with biotinylated molecules
- Easier access to desired target
This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.
Propiedades físicas
Sequence contains N-terminal 6×-Histidine-tag and a C-terminal FLAG-tag
Reconstitución
Once reconstituted, the solution can be stored at 2-8 °C for at least 1 month. For long term storage, aliquot and store at -20 °C.
This product is lyophilized from PBS, pH 7.4. Reconstitute the vial contents to a 4 mg/mL solution by adding water as follows:
The reconstituted product can be further diluted in water or PBS as needed.
- 1 mg vial: add 250 μL of water for a 4 mg/mL solution
- 5 mg vial: add 1.25 mL of water for a 4 mg/mL solution
The reconstituted product can be further diluted in water or PBS as needed.
Información legal
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
SAvPhire is a trademark of Sigma-Aldrich Co. LLC
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
Lot/Batch Number
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Los clientes también vieron
Expression and purification of soluble monomeric streptavidin in Escherichia coli
Demonte D, et al.
Applied Microbiology and Biotechnology, 98(14), 6285-6295 (2014)
Functional expression of monomeric streptavidin and fusion proteins in Escherichia coli: applications in flow cytometry and ELISA.
Kroetsch, A. et al.
Applied Microbiology and Biotechnology, 102, 10079-10089 (2018)
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Could you provide the molar extinction coefficient for this product?
1 answer-
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What is the isoelectric point (pI) of Product No. SAE0094, SAvPhire™ Monomeric Streptavidin?
1 answer-
The isoelectric point (pI) of Product No. SAE0094, SAvPhire™ Monomeric Streptavidin, is not determined by us. However, the theoretical pI is 5.9.
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