Saltar al contenido
Merck
Todas las fotos(3)

Key Documents

View All Documentation

P9599

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases, for plant cell and tissue extracts, DMSO solution

Sinónimos:

Protease Inhibitor Mix

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(3)

About This Item

MDL number:
MFCD00677817
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Cóctel de inhibidores de proteasas, for plant cell and tissue extracts, DMSO solution

Quality Level

300

form

DMSO solution

storage temp.

−20°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Categorías relacionadas

General description

Our Protease Inhibitor Cocktail is a mixture of individual components optimized and tested for use with plant tissue and cell extracts. The cocktail contains a broad specificity of inhibitory properties including serine, cysteine, aspartic and metalloproteases, and aminopeptidases. The individual components include AEBSF, 1,10-Phenanthroline, Pepstatin A, Leupeptin, Bestatin, and E-64.

Specificity

Inhibits serine, cysteine, and aspartic proteases, metalloproteases, and aminopeptidases

Application

This product has been specifically tested on plant seedling extracts from pea, bean, wheat, tobacco, and Arabidopsis, as well as leaf and root extracts from pea, wheat, and tobacco. The recommended quantity is one mL of solution for the inhibition of protease activity in 100 mL of cell lysate from 30 g of various plant tissues or 10 g of baculovirus-infected cells.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF, 1,10-Phenanthroline, Pepstatin A, Leupeptin, Bestatin, and E-64. Each component has specific inhibitory properties. AEBSF acts to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases. 1,10-Phenanthroline acts against metalloproteases.

Features and Benefits

Contains a broad specificity of inhibitory properties including serine, cysteine, aspartic and metalloproteases, and aminopeptidases

Optimized and tested for use with plant tissue and cell extracts

Specifically tested on plant seedling extracts from pea, bean, wheat, tobacco, and Arabidopsis, as well as leaf and root extracts from pea, wheat, and tobacco

One mL of solution is recommended for the inhibition of protease activity in 100 mL of cell lysate from 30 g of various plant tissues or 10 g of baculovirus-infected cells

Components

AEBSF
Bestatin
E-64
Leupeptin
Pepstatin A
1,10-Phenanthroline

Caution

The cocktail should be stored at -20°C, where it will retain stability for two years.

Quantity

One mL is recommended for the inhibition of proteases extracted from 30 g of plant tissue in a total volume of 100 ml.

Preparation Note

This product is supplied as a clear, faint pink solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 30 g of various plant tissues or 10 g of baculovirus-infected cells. Extracts of plant seedlings from pea, bean, wheat, tobacco, and Arabidopsis have been tested. The roots of these plants have also been successfully tested.

related product

Referencia del producto
Descripción
Precios

P8465

Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder

P8340

Cóctel de inhibidores de proteasas, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Cóctel de inhibidores de proteasas, for use with fungal and yeast extracts, DMSO solution

P2714

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

P8849

Cóctel de inhibidores de proteasas, for use in purification of Histidine-tagged proteins, DMSO solution

P1860

Cóctel de inhibidores de proteasas, for use in tissue culture media, DMSO solution

S8820

Comprimidos de inhibidor de proteasas SIGMAFAST™, For General Use

S8830

Comprimidos de cóctel de inhibidores de proteasas SIGMAFAST™ sin EDTA, for use in purification of Histidine-tagged proteins

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

185.0 °F - closed cup

flash_point_c

85 °C - closed cup

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Inkar Castellanos-Huerta et al.
Avian diseases, 60(4), 784-791 (2016-12-03)
Globally, avian influenza (AI) is a serious problem in poultry farming. Despite vaccination, the prevalence of AI in México highlights the need for new approaches to control AI and to reduce the economic losses associated with its occurrence in susceptible
Ullrich Dubiella et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(21), 8744-8749 (2013-05-08)
In animals and plants, pathogen recognition triggers the local activation of intracellular signaling that is prerequisite for mounting systemic defenses in the whole organism. We identified that Arabidopsis thaliana isoform CPK5 of the plant calcium-dependent protein kinase family becomes rapidly
Julia A Chekanova et al.
Cell, 131(7), 1340-1353 (2007-12-28)
The exosome complex plays a central and essential role in RNA metabolism. However, comprehensive studies of exosome substrates and functional analyses of its subunits are lacking. Here, we demonstrate that as opposed to yeast and metazoans the plant exosome core
Birgit Agne et al.
The Journal of biological chemistry, 284(13), 8670-8679 (2009-02-04)
The heterotrimeric Toc core complex of the chloroplast protein import apparatus contains two GTPases, Toc159 and Toc34, together with the protein-conducting channel Toc75. Toc159 and Toc34 are exposed at the chloroplast surface and function in preprotein recognition. Together, they have
Shugo Maekawa et al.
Frontiers in plant science, 9, 1177-1177 (2018-09-14)
The Brix domain is a conserved domain in several proteins involved in ribosome biogenesis in yeast and animals. In the Arabidopsis genome, six Brix domain-containing proteins are encoded; however, their molecular functions have not been fully characterized, as yet. Here
Ver todos los artículos relacionados

Contenido relacionado

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico