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I3786

Sigma-Aldrich

Protease Inhibitor Cocktail

Animal-Free, DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use with mammalian cell and tissue extract, DMSO solution

Sinónimos:

Protease inhibitor

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.77
Formulario:
DMSO solution
temp. de almacenamiento:
−20°C

Nombre del producto

Protease Inhibitor Cocktail, Animal Component Free, for use with mammalian cell and tissue extract, DMSO solution

Nivel de calidad

200

Formulario

DMSO solution

temp. de almacenamiento

−20°C

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Descripción general

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts.

Especificidad

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.

Aplicación

Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted.

Componentes

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Forma física

The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins.
This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 108 cells per mL.

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Referencia del producto
Descripción
Precios

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H315,H319

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
0000231061
0000231059
0000198872
0000198871
029M4158V

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Molecular medicine reports, 16(1), 937-942 (2017-06-07)
The role of inflammation in tumorigenesis and development is currently well established. Lymphotoxin β receptor (LTβR) activation induces canonical and noncanonical nuclear factor (NF)‑κB signaling pathways, which are linked to inflammation‑induced carcinogenesis. In the present study, 5,637 bladder cancer cells were
Yue Gao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 129, 110396-110396 (2020-06-25)
Systemic lupus erythematosus (SLE) is an autoimmune disease that is mainly caused by excessive accumulation of autoantibodies that target autoantibodies such as nucleic acids. T helper (Th) cell have been associated with the development of SLE. Typically, different subsets of
Douglas Esteves Teixeira et al.
Biochimica et biophysica acta. Molecular basis of disease, 1866(11), 165907-165907 (2020-08-02)
High salt diet (HSD), considered a public health problem worldwide, is associated with chronic degenerative diseases including renal diseases. However, little is known about the effects of HSD on renal function independently of the development of hypertension. To address the
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Toxicology in vitro : an international journal published in association with BIBRA, 29(5), 819-827 (2015-03-21)
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Contenido relacionado

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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