Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein.
Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64).
This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1).
Immunogen
Human
a recombinant protein consisting of human 53BP1 fused to a His•Tag sequence
Application
Immunoblotting (1:3000-1:5000)
Immunofluorescence (1:300-1:500, indirect)
Immunoprecipitation (1:300-1:500)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Analysis Note
Positive Control HeLa Cell Nuclear Extract (Cat. No. WB64)
Other Notes
Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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