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M3942

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DMEM - high glucose

MegaCell™, with sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell cutlure

Sinónimos:

DMEM, DMEM Supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

MegaCell™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

Quality Level

300

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
glucose: 4500 mg/L (Dextro)
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

General description

MegaCell™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.

Application

MegaCell™ Dulbecco′s Modified Eagle′s Medium has been used:
  • as a component of the M10 medium to culture CD133+ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
  • as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
  • to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies

Reconstitution

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Legal Information

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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The BCNT (Bucentaur) superfamily is classified by an uncharacteristic conserved sequence of ∼80 amino acids (aa) at the C-terminus, BCNT-C (the conserved C-terminal region of Bcnt/Cfdp1). Whereas the yeast Swc5 and Drosophila Yeti homologues play crucial roles in chromatin remodelling

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