Documentos clave

Ver Toda la documentación

Saltar a

D5671

DMEM - high glucose

Name: DMEM - high glucose
Brand: SIGMA

With sodium Bicarbonate, without ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

DME, DMEM

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas

Tamaño de envase	SKU	Disponibilidad	Precio

Acerca de este artículo

UNSPSC Code:

12352207

NACRES:

NA.75

En este momento no podemos mostrarle ni los precios ni la disponibilidad

Servicio técnico

¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.

Permítanos ayudarle

Propiedades

Nombre del producto

Medio de Eagle modificado de Dulbecco, glucosa elevada, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
glucose: high
NaHCO₃: yes
sodium pyruvate: no
phenol red: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Descripción

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application

El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».

Preparation Note

Supplement with 0.584 g/L L-glutamine.

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo	D5796	D6546	D6429
Sigma-Aldrich D5671 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D5796 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D6546 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D6429 Dulbecco′s Modified Eagle′s Medium - high glucose
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components L-glutamine: no glucose: high NaHCO₃: yes sodium pyruvate: no phenol red: yes HEPES: no	components NaHCO₃: yes L-glutamine: yes glucose: high phenol red: yes sodium pyruvate: no HEPES: no	components NaHCO₃: yes phenol red: yes sodium pyruvate: yes HEPES: no L-glutamine: no glucose: high	components HEPES: no NaHCO₃: yes phenol red: yes sodium pyruvate: yes L-glutamine: yes glucose: high
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

Still not finding the right product?

Explore all of our products under Medio de Eagle modificado de Dulbecco, glucosa elevada

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Protocolos

Protocolo del análisis de la viabilidad y la proliferación celulares con MTT

Protocolo del ensayo con MTT para medir la viabilidad, la proliferación y la citotoxicidad celulares. Instrucciones para la preparación de los reactivos MTT y ejemplos de aplicaciones.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

Ver todos los protocolos

Artículos

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.

Contenido relacionado

Dulbecco Modified Eagle Medium (DME) - Japanese version

Dulbecco Modified Eagle Medium (DME)

Instructions

Cell Culture Essentials Promotions

Learning time-varying information flow from single-cell epithelial to mesenchymal transition data.

Smita Krishnaswamy et al.

PloS one, 13(10), e0203389-e0203389 (2018-10-30)

Cellular regulatory networks are not static, but continuously reconfigure in response to stimuli via alterations in protein abundance and confirmation. However, typical computational approaches treat them as static interaction networks derived from a single time point. Here, we provide methods

Attaching and effacing (A/E) lesion formation by enteropathogenic E. coli on human intestinal mucosa is dependent on non-LEE effectors.

Massiel Cepeda-Molero et al.

PLoS pathogens, 13(10), e1006706-e1006706 (2017-10-31)

Enteropathogenic E. coli (EPEC) is a human pathogen that causes acute and chronic pediatric diarrhea. The hallmark of EPEC infection is the formation of attaching and effacing (A/E) lesions in the intestinal epithelium. Formation of A/E lesions is mediated by

Soluble Neuregulin1 Down-Regulates Myelination Genes in Schwann Cells.

Marwa El Soury et al.

Frontiers in molecular neuroscience, 11, 157-157 (2018-06-06)

Peripheral nerves are characterised by the ability to regenerate after injury. Schwann cell activity is fundamental for all steps of peripheral nerve regeneration: immediately after injury they de-differentiate, remove myelin debris, proliferate and repopulate the injured nerve. Soluble Neuregulin1 (NRG1)

Ver todos los artículos relacionados

Número de artículo de comercio global

SKU	GTIN
D5671-24X500ML	04061833586594
D5671-1L	04061833586587
D5671-20L	04061837340437
D5671-6X500ML	04061833586624
D5671-10L	04061837340420
D5671-6X1L	04061833586617
D5671-500ML	04061833586600

Questions

Reviews

No rating value