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Merck

L2025

Sigma-Aldrich

Luria Agar Base, Miller

Tryptone, yeast extract, sodium chloride and agar

Sinónimos:

LA, modified, Luria-Bertani media, modified, Lysogeny broth, modified

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About This Item

UNSPSC Code:
41106200
eCl@ss:
42040102
NACRES:
NA.72

Quality Level

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

General description

Luria Bertani (LB) medium is one of the complex media that is widely used to grow Escherichia coli in small-scale cultures.

Application

Luria Agar Base, Miller has been used as a growth medium during plasmid amplification. It has also been used as a culture medium for strains of Penicillium sp. laika.
Luria broth/agar (LA), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) yeast extract (5g/L) and agar (15g/L) used to culture members of the Enterobacteriaceae family and for coliphage plaque assays.

Components

Contains: Tryptone, yeast extract, sodium chloride and agar.

Reconstitution

Recommended use at 30.5 g per liter.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Shake-flask cultures are widely used for screening of high producing strains. To select suitable strains for production scale, cultivation parameters should be applied that provide optimal growth conditions. A novel method of measuring respiratory activity in shake-flask cultures was employed

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