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H3537

Sigma-Aldrich

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Sinónimos:

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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100 ML
$70.000
500 ML
$206.000
1 L
$351.000

$70.000

Fecha estimada de envío13 de mayo de 2025

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100 ML
$70.000
500 ML
$206.000
1 L
$351.000

About This Item

Número de CAS:
7365-45-9
Número MDL:
MFCD00006158
Código UNSPSC:
12161700
ID de la sustancia en PubChem:
329815199
NACRES:
NA.25

$70.000

Fecha estimada de envío13 de mayo de 2025

Solicitar un pedido a granel

Nombre del producto

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

grado

BioPerformance Certified

Nivel de calidad

200

esterilidad

0.2 μm filtered

Formulario

liquid

concentración

1 M

técnicas

cell culture | mammalian: suitable

impurezas

Bioburden, tested
 DNase, RNase, Protease, Nickase, free
 endotoxin, tested

pH

5.0-6.0

intervalo de pH útil

6.8-8.2

trazas de catión

Fe: <5 ppm
heavy metals (as Pb): <5 ppm

idoneidad

suitable for molecular biology

aplicaciones

diagnostic assay manufacturing

cadena SMILES

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

Clave InChI

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Aplicación

RNAse HEPES has been used:
  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets[3]
  • To recover purified ribonucleotide[4]
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing[5]
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets[6]

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000417229
0000417234
0000417239
0000417234
0000417239

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Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, et al.
The Journal of Biological Chemistry, 280(38), 32835-32842 (2005)
Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, et al.
Theriogenology, 108, 29-38 (2018)
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria.
Silas S, et al.
Bio-protocol, 8(4) (2018)
Masahiro Sato et al.
International journal of molecular sciences, 16(8), 17838-17856 (2015-08-08)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of
Masahiro Sato et al.
Theriogenology, 108, 29-38 (2017-12-02)
Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related mRNA components into the porcine zygote has been described. Given the relatively long gestational period and the high cost of housing swine, the establishment of an effective microinjection-based porcine genome editing
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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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