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H6147

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sinónimos:

Ácido 4-(2-hidroxietil)piperacina-1-etanosulfónico, Ácido N-(2-hidroxietil)piperacina-N′-(2-etanosulfónico)

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Talla/SKUDisponibilidadPrecio
25 g
Comprobar disponibilidad del carrito
$101.000
100 g
Comprobar disponibilidad del carrito
$305.000
500 g
Comprobar disponibilidad del carrito
$1.070.000

Acerca de este artículo

Fórmula empírica (notación de Hill):
C8H18N2O4S
Número CAS:
Peso molecular:
238.30
UNSPSC Code:
12161704
NACRES:
NA.75
PubChem Substance ID:
EC Number:
230-907-9
Beilstein/REAXYS Number:
883043
MDL number:

$101.000


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product line

BioXtra

Quality Segment

assay

≥99.5% (titration)

form

powder

technique(s)

cell culture | embryo: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

absorption

≤0.05 at 290 in H2O at 33%

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

General description

Useful pH range: 6.8 - 8.2
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Application

HEPES has been used:
  • As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
  • As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
  • in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.
This product has been qualified for use in cell and mouse embryo culture.

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Este artículo
RDD002PHR1428H4034
HEPES Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1428

HEPES

description

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

description

anhydrous, free-flowing, Redi-Dri™, ≥99.5%

description

Pharmaceutical Secondary Standard; Certified Reference Material

description

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

assay

≥99.5% (titration)

assay

≥99.5%

assay

-

assay

≥99.5% (titration)

technique(s)

cell culture | embryo: suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

competitive inhibition ELISA: suitable, cell culture | mammalian: suitable

Quality Level

400

Quality Level

100

Quality Level

300

Quality Level

400

product line

BioXtra

product line

Redi-Dri™

product line

-

product line

-

form

powder

form

crystalline powder

form

-

form

crystalline powder

pH

5.0-6.5 (25 °C, 238 g/L)

pH

5.0-6.5 (25 °C, 238 g/L)

pH

5.0-6.5 (25 °C, 238 g/L)

pH

-


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Clase de almacenamiento

11 - Combustible Solids

¿Qué está pasando?

WGK 1

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

EPI (equipos de protección individual)

Eyeshields, Gloves, type N95 (US)



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