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F7425

Millipore

ANTI-FLAG® antibody produced in rabbit

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-ddddk, Anti-dykddddk

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0.2 MG
$801.000

$801.000

Fecha estimada de envío25 de abril de 2025

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0.2 MG
$801.000

About This Item

Número MDL:
MFCD02262912
Código UNSPSC:
12352203
NACRES:
NA.32

$801.000

Fecha estimada de envío25 de abril de 2025

Solicitar un pedido a granel

origen biológico

rabbit

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

reactividad de especies

all

concentración

~0.8 mg/mL

técnicas

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotipo

IgG

secuencia del inmunógeno

DYKDDDDK

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

El anticuerpo policlonal de afinidad anti-FLAG de conejo ANTI-FLAG reconoce el epítopo FLAG situado en las proteínas de fusión FLAG. Este anticuerpo reacciona con los extremos N-terminal, Met-N-terminal, C-terminal de las proteínas de fusión FLAG.
El epítopo FLAG es una proteína de ocho aminoácidos con un sitio de escisión por enterocinasa.[1][2]

Inmunógeno

FLAG; secuencia peptídica DYKDDDDK

Aplicación

El anticuerpo reconoce el epítopo FLAG situado en las proteínas de fusión etiquetadas con FLAG en los extremos N-terminal o C-terminal mediante la aplicación de ensayos de inmunotransferencia por puntos (dot blot), inmunotransferencia[3][4], inmunoprecipitación[3] e inmunocitoquímica.
Conozca más detalles del producto en nuestro portal de la aplicación FLAG®.

Acciones bioquímicas o fisiológicas

El epítopo FLAG desempeña un papel crucial en la purificación mediante inmunoafinidad de las proteínas de fusión.[1]

Forma física

Disolución en disolución salina tamponada con fosfato 0,01 M, pH 7,4, que contiene seroalbúmina bovina al 1 % y acida sódica 15 mM.

Nota de preparación

Purificado mediante cromatografía de afinidad en una columna portadora del péptido inmunizante.

Otras notas

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

Información legal

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000395474
0000395474
0000252651
0000236831
0000160280

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Ryota Sato et al.
Frontiers in microbiology, 10, 1857-1857 (2019-08-29)
The envelope proteins of influenza A virus, hemagglutinin (HA) and neuraminidase (NA), play critical roles in viral entry to host cells and release from the cells, respectively. After protein synthesis, they are transported from the trans-Golgi network (TGN) to the
Martin S Taylor et al.
The Journal of biological chemistry, 288(45), 32211-32228 (2013-09-21)
Ghrelin O-acyltransferase (GOAT) is a polytopic integral membrane protein required for activation of ghrelin, a secreted metabolism-regulating peptide hormone. Although GOAT is a potential therapeutic target for the treatment of obesity and diabetes and plays a key role in other
Makoto Miyazawa et al.
The Journal of biological chemistry, 286(22), 19191-19203 (2011-04-12)
The molecular chaperone prefoldin (PFD) is a complex comprised of six different subunits, PFD1-PFD6, and delivers newly synthesized unfolded proteins to cytosolic chaperonin TRiC/CCT to facilitate the folding of proteins. PFD subunits also have functions different from the function of
Ecco Staller et al.
Journal of virology, 93(17) (2019-06-21)
ANP32 proteins have been implicated in supporting influenza virus replication, but most of the work to date has focused on the ability of avian Anp32 proteins to overcome restriction of avian influenza polymerases in human cells. Using a CRISPR approach
Dorothee A Vogt et al.
PLoS pathogens, 9(4), e1003302-e1003302 (2013-04-18)
The nonstructural protein NS5A has emerged as a new drug target in antiviral therapies for Hepatitis C Virus (HCV) infection. NS5A is critically involved in viral RNA replication that takes place at newly formed membranes within the endoplasmic reticulum (membranous
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1 Review This action will navigate to reviews.

Questions

1–9 of 9 Questions  

  1. Can this AB (F7425) used for mouse 30um thick section. mouse will be perfused with 4% PFA and than cryopreserved. thanks

    1 answer

    1. This product has been recommended for use in immunofluorescence and indirect immunofluorescence at 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK. It has not been tested for use in IHC applications. The end-user will need to optimize the assay. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/207/353/f7425dat-ms.pdf

      In general, antibodies work well on 5-15 uM sections, for 30 uM sections, blocking /permeabilization steps and antibody dilution, the incubation time will need to be optimized by end user. Please see the links below to review an alternative anti-Flag antibody that may be more suitable for this application. Note that this product is a mouse monoclonal antibody:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
      https://www.sigmaaldrich.com/product/sigma/f1804

      Helpful?

  2. Is there a recommended isotype control for this antibody?

    1 answer

    1. Any rabbit IgG such as product I5006 would be an appropriate isotype control for this antibody.

      Helpful?

  3. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    1 answer

    1. To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

      Helpful?

  4. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    1 answer

    1. For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

      Helpful?

  5. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    1 answer

    1. K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The concentration is 0.9 mg protein per ml.

      Helpful?

  8. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

      Helpful?

  9. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    1 answer

    1. F7425 is a polyclonal antibody from rabbit.

      Helpful?

Reviews

Active Filters

  1. Nottingham
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Western blotting

    I HAVE USED THIS ANTIBODY ALOT DURING MY RESEARCH. AND IT HAS WORKED PERFECTLY.

    Helpful?

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