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Merck

D7876

Sigma-Aldrich

Diamine Oxidase from porcine kidney

≥0.05 unit/mg solid

Sinónimos:

Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)

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250 MG
$71.000
1 G
$228.000
5 G
$688.000
10 G
$1.200.000

$71.000


Fecha estimada de envío24 de abril de 2025


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250 MG
$71.000
1 G
$228.000
5 G
$688.000
10 G
$1.200.000

About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

$71.000


Fecha estimada de envío24 de abril de 2025


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origen biológico

Porcine kidney

Nivel de calidad

Formulario

solid

actividad específica

≥0.05 unit/mg solid

mol peso

170 kDa

solubilidad

100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL

actividad extraña

monoamine oxidase (benzylamine substrate) ≤1%

temp. de almacenamiento

−20°C

Descripción general

Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper.[1] The molecular mass of the enzyme is found to be 170 kDa.[2] The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A.[3] Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.[1]

Aplicación

Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.[4]
Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.

Acciones bioquímicas o fisiológicas

Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide.[5] The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism.[6] Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.[7]

Definición de unidad

One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Diamine oxidase and catalase are expressed in the same cells but are present in different subcellular compartments in porcine kidney.
H G Schwelberger et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 48 Suppl 1, S81-S82 (1999-06-01)
A Rinaldi et al.
Preparative biochemistry, 12(1), 11-28 (1982-01-01)
Several methods for the isolation of apparently homogeneous pig kidney diamine oxidase have been reported in recent years (1-7), but these procedures allow to obtain only little amounts of material making very difficult the study of the molecular properties of
Y Huang et al.
Carbohydrate research, 323(1-4), 111-125 (2000-04-27)
Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The
Y Wang et al.
Analytical biochemistry, 287(2), 299-302 (2000-12-09)
A sensitive chemiluminescence-based method for the assay of ornithine decarboxylase (ODC) has been developed. This method, which permits the detection of putrescine (the product of ODC) at a picomolar range, can be used to determine ODC activity in cellular extracts.
HISTAMINE BIOSENSOR: A REVIEW
NorazlinaOthman F, et al.
The Malaysian Journal of Analytical Sciences (2006)

Protocolos

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

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