D7876
Diamine Oxidase from porcine kidney
≥0.05 unit/mg solid
Sinónimos:
Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)
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250 MG
$71.000
1 G
$228.000
5 G
$688.000
10 G
$1.200.000
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250 MG
$71.000
1 G
$228.000
5 G
$688.000
10 G
$1.200.000
About This Item
Productos recomendados
origen biológico
Porcine kidney
Formulario
solid
actividad específica
≥0.05 unit/mg solid
mol peso
170 kDa
solubilidad
100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL
actividad extraña
monoamine oxidase (benzylamine substrate) ≤1%
temp. de almacenamiento
−20°C
Descripción general
Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper.[1] The molecular mass of the enzyme is found to be 170 kDa.[2] The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A.[3] Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.[1]
Aplicación
Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.[4]
Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.
Acciones bioquímicas o fisiológicas
Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide.[5] The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism.[6] Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.[7]
Definición de unidad
One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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M A Shah et al.
The Biochemical journal, 253(1), 103-107 (1988-07-01)
Pig kidney diamine oxidase (DAO) was found to contain 5% (w/w) natural hexose, 3.25% glucosamine, 2.61% N-acetylglucosamine and 0.25% N-acetylneuraminic acid. The enzyme exhibited strong affinity towards concanavalin A (Con A) with a stoichiometry of 1:4.6. The kinetics of interaction
Marta Moniente et al.
Food research international (Ottawa, Ont.), 160, 111735-111735 (2022-09-10)
Lentilactobacillus parabuchneri is the main bacteria responsible for the accumulation of histamine in cheese. The goal of this study was to assess the efficiency of potential histamine-degrading microbial strains or, alternatively, the action of the diamine oxidase (DAO) enzyme in
Diamine oxidase and catalase are expressed in the same cells but are present in different subcellular compartments in porcine kidney.
H G Schwelberger et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 48 Suppl 1, S81-S82 (1999-06-01)
A Rinaldi et al.
Preparative biochemistry, 12(1), 11-28 (1982-01-01)
Several methods for the isolation of apparently homogeneous pig kidney diamine oxidase have been reported in recent years (1-7), but these procedures allow to obtain only little amounts of material making very difficult the study of the molecular properties of
Y Huang et al.
Carbohydrate research, 323(1-4), 111-125 (2000-04-27)
Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The
Protocolos
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
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