The enzyme may be reconstituted at 1 mg/ml in 10 mM sodium acetate buffer, pH 7.5 with 5 mM calcium acetate. Historical data also note that this material may be reconstituted in PBS or other physiologic buffers containing Ca2+, culture medium, or water; however, no concentration is provided.
D4818
Dispase I
protease
Sinónimos:
Proteasa from Bacillus polymyxa
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About This Item
Productos recomendados
Quality Level
form
lyophilized solid
specific activity
≥10 unit/mg solid
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
storage temp.
2-8°C
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Application
Suitable for use in preparation of single cell suspension for sequencing.
Biochem/physiol Actions
Unit Definition
Physical form
Legal Information
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Certificados de análisis (COA)
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Artículos
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
Contenido relacionado
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
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How to disolve dispase? Can I use mediums as DMEM, PBS ...?
1 answer-
Helpful?
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What is the activity of the Product D4818, Dispase, which I received?
1 answer-
Each lot will have a specific activity that can be found on our website on the certificate of analysis.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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At what concentration should Product D4818, Dispase, be used?
1 answer-
To dissociate cells from ECM (extracellular matrix) use at a concentration of 0.6 - 2.4 units/ml.
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What is the difference between Product D4693, Dispase II and Product D4818, Dispase I?
1 answer-
Product D4693 is a lyophilized powder containing calcium acetate and milk sugar as a stabilizer. The activity specification is >0.5 unit/mg solid. Product D4818 does not contain any milk sugar and has an activity specification of >10 units/mg solid.
Helpful?
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What is the solubility of Product D4818, Dispase I?
1 answer-
The enzyme may be reconstituted at 1 mg/ml in 10 mM sodium acetate buffer, pH 7.5 with 5 mM calcium acetate.
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