3HBDB-RO

3-Hydroxybutyrate Dehydrogenase (3-HBDH)

suspension, ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II

• Sinónimos: 3-Hydroxybutyrate Dehydrogenase (3-HBDH), 3-HBDH
• Comisión internacional de enzimas: 1.1.1.30 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• Nivel de calidad: 100
• Formulario: suspension
• actividad específica: ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
• envase: vial of 2 mL (10127833001); vial of 5 mL (10127841001)
• fabricante / nombre comercial: Roche
• concentración: 5 mg/mL
• impurezas: <0.1% LDH; <5% MDH
• pH óptimo: 6.2-6.9(for reduction); 7-9(for oxidation)
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.[1]

Aplicación

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.

Acciones bioquímicas o fisiológicas

3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-­hydroxybutyrate.[2] In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.[3]

Calidad

Contaminants: <0.1% LDH, <5% MDH

Forma física

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Nota de preparación

Stabilizers: NADH, Ca²⁺

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): No data available
• Punto de inflamabilidad (°C): No data available