Tyrosinase is soluble (2 mg/mL) in 50 mM potassium phosphate buffer, pH 6.5, yielding a clear brown solution.
The use of other buffers, such as phosphate buffer has not been evaluated. For optimal results and to minimize the risk of issues, we recommend closely following the provided instructions.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/407/287/t3824dat-mk.pdf
T3824
Tirosinasa from mushroom
lyophilized powder, ≥1000 unit/mg solid
Tyrosinase from mushroom
Sinónimos:
Catecoloxidasa, Monofenol monooxigenasa, Monofenol, dihidroxifenilalanina:oxígeno oxidorreductasa, Polifenol oxidasa
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
25000 units | Comprobar disponibilidad del carrito | $158.000 |
50000 units | Comprobar disponibilidad del carrito | $271.000 |
250000 units | Comprobar disponibilidad del carrito | $826.000 |
500000 units | Comprobar disponibilidad del carrito | $1.240.000 |
1000000 units | Comprobar disponibilidad del carrito | $1.940.000 |
Acerca de este artículo
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥1000 unit/mg solid
Biological source:
fungus (mushroom)
Servicio técnico
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Permítanos ayudarlebiological source
fungus (mushroom)
Quality Segment
form
lyophilized powder
specific activity
≥1000 unit/mg solid
mol wt
119.5 kDa by electrophoresis, 128 kDa by sedimentation velocity diffusion, 133 kDa by LS
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
La tirosinasa es una oxidasa que contiene cobre con actividad para catecoles y cresol. La tirosinasa participa en la conversión de la tirosinasa a dihidroxifenilalanina (DOPA). La polimerización de la DOPA lleva a la formación de melanina. La tirosinasa desempeña un papel en la reacciones del bronceado. Se dice que la enzima tiene dos sitios de unión para sustratos aromáticos y un sitio de unión aparte para oxígeno-cobre.
Punto isoeléctrico (pI) 4,7-5
El pH óptimo es 6-7
Peso molecular: 128 kDa por difusión de velocidad de sedimentación; 133 kDa mediante medición de dispersión de la luz y 119,5 kDa por electroforesis.
La tirosinasa es una oxidasa que contiene cobre, con actividad para catecoles y cresol. Es responsable de las reacciones de pardeamiento. Se ha comunicado que la enzima tiene dos sitios de unión para sustratos aromáticos y un sitio de unión diferente para oxígeno-cobre. El cobre es probablemente el Cu(I), y su inactivación implicará la oxidación a Cu(II).
El pH óptimo es 6-7
Peso molecular: 128 kDa por difusión de velocidad de sedimentación; 133 kDa mediante medición de dispersión de la luz y 119,5 kDa por electroforesis.
La tirosinasa es una oxidasa que contiene cobre, con actividad para catecoles y cresol. Es responsable de las reacciones de pardeamiento. Se ha comunicado que la enzima tiene dos sitios de unión para sustratos aromáticos y un sitio de unión diferente para oxígeno-cobre. El cobre es probablemente el Cu(I), y su inactivación implicará la oxidación a Cu(II).
Punto isoeléctrico (pI) 4,7-5
El pH óptimo es 6-7
Peso molecular: 128 kDa por difusión de velocidad de sedimentación; 133 kDa mediante medición de dispersión de la luz y 119,5 kDa por electroforesis.
El pH óptimo es 6-7
Peso molecular: 128 kDa por difusión de velocidad de sedimentación; 133 kDa mediante medición de dispersión de la luz y 119,5 kDa por electroforesis.
Application
La actividad tirosinasa se ha evaluado en un estudio en el que se desarrollaba un agente terapéutico alternativo para el tratamiento de la hiperpigmentación. [1] La tirosinasa también se ha utilizado en un estudio en el que se investigaba el fenotipo de albinismo oculocutáneo en la población paquistaní. [2]
Biochem/physiol Actions
Convierte la tirosina en L-DOPA o la tiramina a dopamina
Other Notes
Una unidad = ΔA280 de 0,001 por minuto a pH 6,5 y 25 °C en 3 ml de mezcla de reacción que contenga L-tirosina.
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Este artículo | |||
|---|---|---|---|
| specific activity ≥1000 unit/mg solid | specific activity ≥20 units/mg protein | specific activity 100,000-250,000 units/g solid (without added oxygen) | specific activity ≥30 units/mg solid |
| biological source fungus (mushroom) | biological source fungus (Trichoderma viride) | biological source - | biological source fungus (Dactylium dendroides) |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| mol wt 119.5 kDa by electrophoresis, 133 kDa by LS, 128 kDa by sedimentation velocity diffusion | mol wt 112 kDa | mol wt 160 kDa | mol wt - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is it possible to use an equivalent buffer, such as phosphate buffer (Na/K), instead of the recommended potassium phosphate buffer to reconstitute the enzyme?
1 answer-
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Is it possible to reconstitute T3824-25KU with a solution other than potassium phosphate buffer?
1 answer-
The enzymatic assays are performed using potassium phosphate buffer as a solvent. Unfortunately, no other solvent was tested in-house. An online search indicates that water may be used to initially reconstitute the enzyme and then further dilute to the solution suitable for the application.
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Does the solid contain any copper specie?
1 answer-
Yes, all native forms of Tyrosinase from mushroom contain Copper. Please see the link below to review additional information regarding the structure and sequence information of Tyrosinase from mushroom.
https://www.rcsb.org/structure/2Y9XHelpful?
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Is this Tyrosinase from mushroom food grade?
1 answer-
This product is not food grade. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals. Currently, a food grade product option is not available.
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When measuring the activity of Tyrosinase, should we use the highest change observed every minute between Tyrosinase and the mixture (with L-tyrosine) as the activity, or the average change over the measured time?
1 answer-
Refer to the protocol of the enzymatic assay of Tyrosinase as described in the reference: Duckworth, H. W. and Coleman, J. E. (1970) Journal of Biological Chemistry, 245, 1613-1625. The protocol can be accessed at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/776/246/tyrosinase.pdf
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Is this tyrosinase is derived from Agaricus bisporus or another mushroom species?
1 answer-
Yes, this product is derived from Agaricus bisporus.
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Can you please tell me 25000 units tyrosinase in milligrams?
1 answer-
This product is sold by activity. The minimum activity specification for this material is 1000 units per milligram. The weight of this product can be determined using the activity value reported on the lot specific Certificate of Analysis(COA). For example, the sample COA has a activity value of 6540 units/mg. The amount of powder in the 25,000 unit package will be 3.82mg.
Please see the link below or navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/t3824#product-documentationHelpful?
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Hi, How much is the total protein amount of T3824 (25KU) per vial?
1 answer-
The protein content of this product is not determined. This product is tested and released based on the activity per milligram solid. Should the protein content be required, please see the link below to review several kits that may be of interest:
https://www.sigmaaldrich.com/US/en/product/compare?term=total%20protein%20kit&facet%5B0%5D=facet_product_category%3Akits&focus=products&page=1&perpage=30&sort=relevance&type=product&products=TP0300%3ASIGMA%3Asial%2CTP0200%3ASIGMA%3Asial%2CTP0100%3ASIGMA%3AsialHelpful?
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What solution is best to be used to reconstitute the T3824-25KU? and what is the volume and concetration of that soluation?
1 answer-
Tyrosinase is soluble at 2 mg/mL in 50 mM potassium phosphate buffer. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/377/683/t3824dat-ms.pdfHelpful?
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I see that the approximate activity of Product T3824, Tyrosinase from mushroom, is ?1000 unit/mg solid, but how can I find the lot specific activity of this tyrosinase?
1 answer-
The certificate of analysis for the lot will report the exact tyrosinase activity for a particular batch. The lot number can be found on the label of the bottle, or you can call technical service (800-325-5832) for a sample lot number to view the online certificate of analysis.
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