Product length is not determined. There is no specific fragmentation step in the preparation of the standards.
S8001M
CpGenome Human Methylated DNA Standard Set
It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
Sinónimos:
CpGenome Methylated DNA, Human DNA Standards, Methylated DNA Standard Set
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About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.77
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Nivel de calidad
reactividad de especies
human
fabricante / nombre comercial
CpGenome
Upstate®
aplicaciones
genomic analysis
Condiciones de envío
dry ice
Descripción general
The CpGenome Human Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. This Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase. It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ amplification kits.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome Human Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome Human Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for the methylated DNA standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome Human Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome Human Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for the methylated DNA standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Aplicación
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Forma física
Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Almacenamiento y estabilidad
Recommended Storage: Stable at -20°C for up to 6 months from date of receipt.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Hello. I wonder the length of methylated dna in this product. Is it a completely entire human genome or fragmented in different dna molecules in different sizes as a mixture. Thank you for your reply in advance.
1 answer-
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