Both D2438 and D2650 are suitable for cell culture. The D2650 is part of the Hybri-Max™ product line. Hybri-Max products have been tested for use in hybridoma creation and culture. The D2438 is part of the BioPerformance Certified group of products. These products are tested and optimized for use in biological applications. For this product and a number of others, this includes testing to meet USP and EP guidelines. Please see the links below to review a sample Certificate of Analysis for each item.
D2650
https://www.sigmaaldrich.com/product/sigma/d2438#product-documentation
D2438
https://www.sigmaaldrich.com/product/sigma/d2650#product-documentation
D2438
Dimetilsulfóxido
Dimethyl sulfoxide
sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sinónimos:
DMSO
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5 X 10 ML
$194.000
50 ML
$262.000
$194.000
Fecha estimada de envío23 de abril de 2025
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5 X 10 ML
$194.000
50 ML
$262.000
About This Item
Fórmula lineal:
(CH3)2SO
Número de CAS:
Peso molecular:
78.13
Beilstein:
506008
Número CE:
Número MDL:
Código UNSPSC:
12191502
ID de la sustancia en PubChem:
NACRES:
NA.75
grado:
BioPerformance Certified
técnicas:
cell culture | hybridoma: suitable
cryopreservation: suitable
cryopreservation: suitable
bp:
189 °C (lit.)
presión de vapor:
0.42 mmHg ( 20 °C)
$194.000
Fecha estimada de envío23 de abril de 2025
Productos recomendados
grado
BioPerformance Certified
Nivel de calidad
Agency
USP XXIV
meets EP testing specifications
meets USP testing specifications
densidad de vapor
2.7 (vs air)
presión de vapor
0.42 mmHg ( 20 °C)
esterilidad
sterile-filtered
Formulario
liquid
temp. de autoignición
573 °F
lim. expl.
42 %, 63 °F
técnicas
cell culture | hybridoma: suitable
cryopreservation: suitable
impurezas
≤0.1% water
color
colorless
índice de refracción
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubilidad
H2O: miscible (completely)
densidad
1.10 g/mL (lit.)
cadena SMILES
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
Clave InChI
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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Descripción general
El dimetilsulfóxido (DMSO) es un disolvente aprótico. Las moléculas terapéuticas y tóxicas que no son solubles en agua suelen ser solubles en DMSO. El nitrógeno líquido utilizado junto con un crioprotector como el DMSO es un método muy utilizado para conservar las células. El DMSO se ha utilizado para preparar disoluciones madre de varias moléculas y también se utiliza como control para dichas moléculas. Se ha utilizado en el ensayo de migración celular para disolver las células después de la migración. El DMSO probada en BPC también cumple los requisitos de las aplicaciones biotecnológicas y de ingeniería tisular.
Aplicación
El dimetilsulfóxido se ha utilizado como vehículo de control para cultivar e inducir la activación de células estrelladas hepáticas (HSC) in vivo.[1][1] También se ha utilizado para preparar una disolución de reserva para estudiar la citotoxicidad del ácido ferúlico (FA) en las células del núcleo pulposo (NP).[2]
Características y beneficios
Producto esterilizado por filtración listo para usar convenientemente envasado en botellas ámbar con membrana de goma y tapón de rosca. Cumple los requisitos analíticos de la USP y la EP
Precaución
Fácilmente superenfriado, vuelve a fundir lentamente a temperatura ambiente. Producto solidificado que puede volver a licuarse por calentamiento a temperatura ambiente sin detrimento del producto.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
188.6 °F - closed cup
Punto de inflamabilidad (°C)
87 °C - closed cup
Equipo de protección personal
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Artículos
Overview of good cell banking practices for cell line cryopreservation purposes.
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Hi, what is the difference between DMSO product# D2438 and D2650? What is Hybri-Max vs. BioPerformance certified and meets USP testing specification?
1 answer-
Helpful?
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Purity grade of the product ?
1 answer-
The purity of this product is listed on the lot specific Certificate of Analysis. Please navigate to the DOCUMENTATION section of the Product Detail Page to access a Certificate:
https://www.sigmaaldrich.com/product/sigma/d2438#product-documentationHelpful?
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El DMSO es sensible a la luz, hay que tomar alguna precaución al respecto?
1 answer-
This product is packaged in amber glass and is protected from light. The 10 mL size is packaged in a sealed ampule. Once opened, any content that is not used should also be stored in amber glass. For long term storage, the material may also be stored under inert gas to preserve the shelf life. Note that this material has a tendency to absorb water. Containers should be tightly sealed.
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what are the storing conditions?
1 answer-
This product is stored at room temperature. Store in a well-ventilated place. Keep cool. Please see the Material Safety Data Sheet for more information: https://www.sigmaaldrich.com/sds/sigma/d2438.
Helpful?
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What is the difference between products D2438 and D8418?
1 answer-
The D2438 is a BioPerformance Certified grade product suitable for cell culture, hybridoma creation, and cryopreservation. It is intended for research use only but does meet USP and EP testing requirements. The D8418 is a molecular biology-grade product suitable for DNA sequencing, PCR, and transfection. It has been tested for RNAse and DNAse. The products can be selected based on the application.
Please view the sample Certificate of Analysis for each product to see the testing specifications associated with that item:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/fluka/pdf/PDF734068.pdf
https://www.sigmaaldrich.com/coa/SIGMA/D2438/RNBL8837Helpful?
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What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)?
1 answer-
Product D2438 is assessed for cell freezing. Cells are frozen and stored at -70°C. The cells are thawed, cultured and viability is assessed at 24 hours. There is no evidence of cytotoxicity or abnormal cell growth.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Where does DMSO come from?
1 answer-
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
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What is the molarity of DMSO?
1 answer-
DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
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What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?
1 answer-
DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.
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