Saltar al contenido
Merck
Todas las fotos(1)

Documentos

D3035

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

Sinónimos:

DNA

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número de CAS:
9007-49-2
MDL number:
MFCD00130921
UNSPSC Code:
41106310
NACRES:
NA.52

biological source

human placenta

grade

for molecular biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Application

Deoxyribonucleic acid from human placenta has been used:
  • to compare the efficiency of DNA quantification methods
  • as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
  • as an internal control to estimate the degree of fragmentation of circulating DNA
  • for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
  • to compare the 70-bp P5 exon sequence between DNA of different human and primate species
  • to calculate copy number of genes, relative to normal human tissue
  • in PCR reactions
For use in Southern hybridizations.

Recommended products

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

related product

Referencia del producto
Descripción
Precios

D4522

Deoxyribonucleic acid from calf thymus, Type XV, Activated, lyophilized powder

D3287

Deoxyribonucleic acid, single stranded from human placenta

D4889

Deoxyribonucleic acid sodium salt from Escherichia coli strain B, Genomic, unsheared

D7290

Deoxyribonucleic acid from herring sperm, single-stranded for hybridization

D7656

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

D9156

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

D3160

Deoxyribonucleic acid from human placenta, buffered aqueous solution, sexed, male

D8661

Deoxyribonucleic acid solution from calf thymus, For hybridization

S8140

SSPE Buffer, for Northern and Southern blotting, powder blend

N6019

Neutralizing Buffer, for Northern and Southern blotting, powder

N1531

Denaturation Solution, for Northern and Southern blotting

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

S2015

SSPE Buffer 20× Concentrate, for Northern and Southern blotting, solution

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

S0902

SSC Buffer, for Northern and Southern blotting, powder blend

H7033

PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

S8015

SSC Buffer, for Northern and Southern blotting, powder blend

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

C7594

Casein Blocking Buffer, for Northern and Southern blotting, powder blend

15356

Blotting-Nylon 66 membranes, type B, positive, for Northern and Southern blotting

31149

Deoxyribonucleic acid, low molecular weight from salmon sperm

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Alain R Thierry et al.
Nucleic acids research, 38(18), 6159-6175 (2010-05-25)
Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an
Joanne S Aveyard et al.
The Journal of molecular diagnostics : JMD, 6(4), 356-365 (2004-10-28)
Many tumors have large homozygous deletions of the CDKN2A locus (encoding p14(ARF) and p16) and of CDKN2B (p15). Our aim was to determine which gene is the major target in bladder cancer. We used quantitative real-time PCR (RTQ-PCR) to determine
Heather Hoover et al.
Journal of proteome research, 14(9), 3670-3679 (2015-07-08)
Tumor types can be defined cytologically by their regions of chromosomal amplification, which often results in the high expression of both mRNA and proteins of certain genes contained within the amplicon. An important strategy for defining therapeutically relevant targets in
Claire E L Smith et al.
PloS one, 12(9), e0185678-e0185678 (2017-09-29)
The imprinted gene PLAGL1 is an important regulator of apoptosis and cell cycle arrest. Loss of its expression has been implicated in tumorigenesis in a range of different cancers, and overexpression during fetal development causes transient neonatal diabetes mellitus (TNDM).
J E Collins et al.
Genome research, 7(5), 522-531 (1997-05-01)
A clone map consisting of YACs, cosmids, and fosmids has been constructed covering low copy repeat regions of human chromosome 22q11. A combination of clone restriction digest analysis, single-copy landmark content analysis, HindIII-Sau3AI fingerprinting, and sequencing of PCR products derived
Ver todos los artículos relacionados

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico