D3035

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

• Sinónimos: DNA
• Número CAS: 9007-49-2
• UNSPSC Code: 41106310
• NACRES: NA.52
• MDL number: MFCD00130921
• eCl@ss: 32160414
• Biological source: human placenta
• Form: buffered aqueous solution
• Storage temp.: −20°C

Propiedades

• biological source: human placenta
• grade: Molecular Biology
• form: buffered aqueous solution
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O
• InChI: 1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)
• InChI key: AWBASQCACWFTGD-UHFFFAOYSA-N

Descripción

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Application

Deoxyribonucleic acid from human placenta has been used:

• to compare the efficiency of DNA quantification methods[1]
• as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines[2]
• as an internal control to estimate the degree of fragmentation of circulating DNA[3]
• for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11[4]
• to compare the 70-bp P5 exon sequence between DNA of different human and primate species[5]
• to calculate copy number of genes, relative to normal human tissue[6]
• in PCR reactions[7]

For use in Southern hybridizations.

Other Notes

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

Información de seguridad

• wgk: WGK 3
• Clase de almacenamiento: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)